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| Publication Type:
| Refereed |
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| Author(s): | Hu, S. L.;
Chai, M. L.;
Guo, D. C.;
Niu, Y. M.;
Dong, L. S. |
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| Author Affiliation: | Inst. of Botany, Beijing, Hangzhou Bot. Garden, Hangzhou, Qingdao Turf. Dev. Co., Jiaozhou |
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| Title: | In vitro clonal propagation of three zoysia species |
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| Meeting Info.: | 7th International Turfgrass Society Research Conference, Palm Beach, FL, USA, 18-24 July, 1993 |
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| Source: | International Turfgrass Society Research Journal. Vol. 7, 1993, p. 812-815. |
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| Publishing Information: | Overland Park, KS: INTERTEC Publishing Corp. |
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| # of Pages: | 4 |
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| Keywords: | TIC Keywords: In vitro; Clones; Tissue culture; Propagation; Zoysia japonica; Zoysia matrella; Zoysia sinica; Calli
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| Abstract/Contents: | "This study involved in vitro clonal propagation of Manila Lawngrass (Zoysia matrella L.), Japanese Lawngrass (Z. japonica Steud.) and Chinese Lawngrass (Z. sinica Hance). Two clones of Manila Lawngrass were selected by means of direct bud multiplication or differentiation of callus induced from stolon explants, while clones of Japanese Lawngrass and Chinese Lawngrass were chosen from seedlings. Murachige and Skoog (MS) callus induction medium was supplemented with 1 t0 5 mg/L 2,4-D (2.4-dichlorophenoxy-acetic acid). Explants were incubated in dark for at least one month. The callus differentiation medium was MS medium with 1 to 2mg/L of 6-BA (6-benzyladenine) and 0 to 0.2 mg/L of IBA (indolebutyric acid). The multiplication medium was MS supplemented with 6-BA(KT,ZT) 1-2mg/ + IBA 0-0.2 mg/L. Shoot clusters of the three species were induced to roots in 1/2MS medium containing IBA 0.5 mg/L, NAA (naphthylaceticacid) 0.5 mg/L, or IBA 0.25 mg/L + NAA 0.25 mg/L. Rooting to 90% for Japanese Lawngrass. The rooted plantlets were transplanted, and their survival rates reached over 95% and 90% for Manila Lawngrass and Japanese Lawngrass, respectively." |
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| Language: | English |
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| References: | 2 |
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| See Also: | Other items relating to: ZOYSIA |
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| Note: | "Chapter 116" |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Hu, S. L., M. L. Chai, D. C. Guo, Y. M. Niu, and L. S. Dong. 1993. In vitro clonal propagation of three zoysia species. Int. Turfgrass Soc. Res. J. 7:p. 812-815. |
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