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Web URL(s):https://www.researchgate.net/publication/317244263_A_rapid_one-step_PCR_protocol_to_distinguish_between_perennial_Lolium_perenne_and_annual_L_multiflorum_ryegrass_seeds
    Last checked: 10/15/2019
    Access conditions: Item is within a limited-access website
Publication Type:
Author(s):Wu, Y. G.; Dong, R.; Luo, D.; Liu, W. X.; Wang, Y. R.; Liu, Z. P.
Author Affiliation:State Key Laboratory of Grassland Agro-ecosystems, School of Pastoral Agricultural Science and Technology, Lanzhou University, Lanzhou, China
Title:A rapid one-step PCR protocol to distinguish between perennial (Lolium perenne) and annual (L. multiflorum) ryegrass seeds
Source:Seed Science and Technology. Vol. 45, No. 2, 2017, p. 444-454.
# of Pages:11
Publishing Information:Zurich, Switzerland: International Seed Testing Association
Related Web URL:http://www.ingentaconnect.com/contentone/ista/sst/2017/00000045/00000002/art00013
    Last checked: 10/03/2017
    Notes: Abstract only
Keywords:TIC Keywords: Comparisons; DNA; Evaluations; Lolium multiflorum; Lolium perenne; Polyacrylamide gels; Polymerase chain reaction; Seed inoculation
Abstract/Contents:"Perennial ryegrass (Lolium perenne) is widely used for both turf cover and forage, whereas annual ryegrass (L. multiflorum) is only used for temporary ground cover because it does not over-winter. Unfortunately, distinguishing seeds of perennial and annual ryegrass from one another is difficult because of their similarities in weight, shape, size and colour. Previous methods to distinguish these seeds have been unreliable and timeconsuming [time-consuming]. Therefore, there is a need for an accurate and low-cost alternative method to distinguish ryegrass species. Here, a one-step protocol that combines polymerase chain reaction (PCR) and polyacrylamide gel electrophoresis (PAGE) techniques was developed to distinguish annual and perennial ryegrass seeds from one another. Based on differences between the DNA sequences in the plastid regions, a pair of primers was designed to distinguish the accessions of 26 perennial and 34 annual ryegrass using this protocol. The results clearly differentiated the two species, confirming the accuracy and sensitivity of this method."
Note:Pictures, b/w
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Wu, Y. G., R. Dong, D. Luo, W. X. Liu, Y. R. Wang, and Z. P. Liu. 2017. A rapid one-step PCR protocol to distinguish between perennial (Lolium perenne) and annual (L. multiflorum) ryegrass seeds. Seed Sci. Technol. 45(2):p. 444-454.
Fastlink to access this record outside TGIF: https://tic.msu.edu/tgif/flink?recno=289638
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DOI: 10.15258/sst.2017.45.2.05
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    Last checked: 10/15/2019
    Access conditions: Item is within a limited-access website
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MSU catalog number: b2224044
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