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| DOI: | 10.1002/ps.4651 |
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| Web URL(s): | http://onlinelibrary.wiley.com/doi/10.1002/ps.4651/full
Last checked: 11/03/2017
Access conditions: Item is within a limited-access website
http://onlinelibrary.wiley.com/doi/10.1002/ps.4651/epdf
Last checked: 11/03/2017
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| Publication Type:
| Refereed |
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| Author(s): | Li, Jian;
Li, Mei;
Gao, Xingxiang;
Fang, Feng |
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| Author Affiliation: | Mei Li: Weed Science Department, Institute of Plant Protection, Sandong Academy of Agricultural Sciences, Jinan, China |
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| Title: | A novel amino acid substitution Trp574Arg in acetolactate synthase (ALS) confers broad resistance to ALS-inhibiting herbicides in crabgrass (Digitaria sanguinalis) |
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| Source: | Pest Management Science. Vol. 73, No. 12, December 2017, p. 2538-2543. |
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| Publishing Information: | Barking, Essex, United Kingdom: Elsevier Science Publishers Ltd. |
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| # of Pages: | 6 |
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| Related Web URL: | http://onlinelibrary.wiley.com/doi/10.1002/ps.4651/abstract
Last checked: 11/03/2017
Notes: Abstract only |
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| Keywords: | TIC Keywords: Acetolactate synthase; Crabgrass control; Digitaria sanguinalis; Evaluations; Genetic markers; Herbicide resistance; Nicosulfuron; Sequence-related amplified polymorphism
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| Abstract/Contents: | "BACKGROUND: Crabgrass (Digitaria sanguinalis) is an annual monocotyledonous weed. In recent years, field applications of nicosulfuron have been ineffective in controlling crabgrass populations in Shandong Province, China. To investigate the mechanisms of resistance to nicosulfuron in crabgrass populations, the acetolactate synthase (ALS) gene fragment covering known resistance-confering mutation sites was amplified and sequenced. RESULTS: Dose-response experiments suggested that the resistant population SD13 (R) was highly resistant to nicosulfuron (resistance index R/S = 43.7) compared with the sensitive population SD22 (S). ALS gene sequencing revealed a Trp574Arg substitution in the SD13 population, and no other known resistance-conferring mutations were found. In vitro ALS enzyme assays further confirmed that the SD13 population was resistant to all tested ALS-inhibiting herbicides. The resistance pattern experiments revealed that, compared with SD22, the SD13 population exhibited broad-spectrum resistance to nicosulfuron (43.7-fold), imazethapyr (11.4-fold) and flumetsulam (16.1-fold); however, it did not develop resistance to atrazine, mesotrione and topramezone. CONCLUSIONS: This study demonstrated that Trp574Arg substitution was the main reason for crabgrass resistance to ALS-inhibiting herbicides. To our knowledge, this is the first report of Trp574Arg substitution in a weed species, and is the first report of target-site mechanisms of herbicide resistance for crabgrass." |
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| Language: | English |
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| References: | 35 |
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| Note: | Pictures, color
Figures
Tables
Graphs |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Li, J., M. Li, X. Gao, and F. Fang. 2017. A novel amino acid substitution Trp574Arg in acetolactate synthase (ALS) confers broad resistance to ALS-inhibiting herbicides in crabgrass (Digitaria sanguinalis). Pest Management Science. 73(12):p. 2538-2543. |
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| DOI: 10.1002/ps.4651 |
| Web URL(s):
http://onlinelibrary.wiley.com/doi/10.1002/ps.4651/full
Last checked: 11/03/2017
Access conditions: Item is within a limited-access website
http://onlinelibrary.wiley.com/doi/10.1002/ps.4651/epdf
Last checked: 11/03/2017
Requires: PDF Reader
Access conditions: Item is within a limited-access webiste |
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MSU catalog number: b2219665 |
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