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| Web URL(s): | http://onlinelibrary.wiley.com/doi/10.1111/j.1744-7348.1993.tb04008.x/epdf
Last checked: 09/29/2015
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Access conditions: Item is within a limited-access website |
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| Publication Type:
| Refereed |
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| Author(s): | Humphreys, I. C.;
Blackshaw, R. P.;
Stewart, R. M.;
Coll, C. |
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| Author Affiliation: | Department of Agricultural Zoology, The Queen's University of Belfast, Newforge Lane; Environmental Sciences Department, The Scottish Agricultural College, Auchincruive; Crop Biology Department, The Scottish Agricultural College, Aberdeen |
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| Title: | Differentiation between larvae of Tipula paludosa and Tipula oleracea (Diptera: Tipulidae) using isoelectric focusing, and their occurrence in grassland in northern Britain |
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| Source: | Annals of Applied Biology. Vol. 122, No. 1, February 1993, p. 1-8. |
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| Publishing Information: | London: Cambridge University Press. |
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| # of Pages: | 8 |
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| Keywords: | TIC Keywords: Distribution; Identification; Isoelectric point; Diptera; Tipula paludosa; Tipulidae; Evaluative methods
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| Geographic Terms: | United Kingdom |
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| Abstract/Contents: | "Thin layer agarose isoelectric focusing (IEF) was used to separate proteins from the larvae of Tipula oleracea Linnaeus and Tipula paludosa Meigen (Diptera, Nematocera). Silver staining revealed protein banding patterns which consistently distinguished these two species at any stage in their life cycles. Within the pH range 5-6 Tipula paludosa contained one major protein band and T. oleracea two bands, with the pI value of the T. paludosa protein being slightly higher than those of T. oleracea. The IEF method is particularly useful for the larval stages of these species because they are often visually inseparable. Leatherjackets obtained during a survey of Northern Ireland and north-eastern Scotland were tested to determine the distribution and frequency of these species occurring in grassland. In each area surveyed T. paludosa predominated with only one field in Northern Ireland and north-eastern Scotland found to contain T. oleracea. In western Scotland T. oleracea occurred in five fields each year of the survey, and was locally abundant on the Isle of Bute in the 1990/91 survey making up 15% of the total larvae tested." |
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| Language: | English |
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| References: | 19 |
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| Note: | Figures
Tables |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Humbphreys, I. C., R. P. Blackshaw, R. M. Stewart, and C. Coll. 1993. Differentiation between larvae of Tipula paludosa and Tipula oleracea (Diptera: Tipulidae) using isoelectric focusing, and their occurrence in grassland in northern Britain. Ann. Appl. Biol. 122(1):p. 1-8. |
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| Web URL(s):
http://onlinelibrary.wiley.com/doi/10.1111/j.1744-7348.1993.tb04008.x/epdf
Last checked: 09/29/2015
Requires: PDF Reader
Access conditions: Item is within a limited-access website |
| 
MSU catalog number: QH 301 .A48 |
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