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| DOI: | 10.1007/s11032-018-0775-4 |
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| Web URL(s): | https://link.springer.com/article/10.1007%2Fs11032-018-0775-4
Last checked: 07/20/2018
Access conditions: Item is within a limited-access website
https://link.springer.com/content/pdf/10.1007%2Fs11032-018-0775-4.pdf
Last checked: 07/20/2018
Requires: PDF Reader
Access conditions: Item is within a limited-access website |
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| Publication Type:
| Refereed |
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| Author(s): | Pan, Ling;
Huang, Ting;
Yang, Zhongfu;
Tang, Lu;
Cheng, Yajuan;
Wang, Jianping;
Ma, Xiao;
Zhang, Xinquan |
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| Author Affiliation: | Pan, Huang, Yang, Tang, Cheng, Ma, and Zhang: Department of Grassland Science, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, China; Wang: Agronomy Department, University of Florida, Gainesville, FL |
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| Title: | EST-SSR marker characterization based on RNA-sequencing of Lolium multiforum and cross transferability to related species |
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| Source: | Molecular Breeding. Vol. 38, No. 6, June 2018, p. 80 [1-12]. |
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| Publishing Information: | The Netherlands: Kluwer Academic Publishers |
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| # of Pages: | 12 |
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| Related Web URL: | https://link.springer.com/article/10.1007%2Fs11032-018-0775-4#Abs1
Last checked: 07/24/2018
Notes: Abstract only |
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| Keywords: | TIC Keywords: Cross-compatibility; Diploids; Drought stress; Genetic markers; Lolium multiflorum; Ribonucleic acid; Simple sequence repeats
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| Abstract/Contents: | "Simple sequence repeat (SSR) markers are considered very useful molecular markers for genetic and molecular breeding, specifically for non-model species. To develop a set of whole genome-wide distributed SSR marker for annual ryegrass (Lolium multiflorum L.), we obtained the first transcriptome dataset of annual ryegrass subjected to drought stress and identified a total of 11,254 EST-SSR. Fifty EST-SSR markers were selected to test the amplification and polymorphism rates in 23 accessions of Lolium and Festuca diploid species. The results showed that all of the 50 functionally relevant markers were polymorphic, with allele number and polymorphism content averaging 7.4 and 0.839, respectively, and fully transferable across 23 accessions of Lolium and Festuca species. Sequencing of PCR products showed that the nucleotide sequences of Lolium and Festuca species had a high level of conservation. Different types of tri-repeats as abundant SSR motifs were observed in repeat sequence regions. A number of EST-SSRs were mapped to genome sequences of Lolium perenne and chromosomes of Brachypodium distachyon. Among them, 973 and 1375 Lolium SSR markers were located on the five chromosomes of B. distachyon and L. perenne scaffolds, respectively. In addition, we confirmed that EST-SSRs as well as SRAP markers could clearly identify varieties of Lolium and Festuca species, and the two sets of molecular markers had a significant correlation (r?=?0.74). This set of EST-SSR markers are valuable genetic tools for genetic diversity, evolutionary, and association mapping studies in Lolium, Festuca, and many other grass species." |
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| Language: | English |
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| References: | 31 |
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| Note: | Figures
Tables
Graphs |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Pan, L., T. Huang, Z. Yang, L. Tang, Y. Cheng, J. Wang, et al. 2018. EST-SSR marker characterization based on RNA-sequencing of Lolium multiforum and cross transferability to related species. Molecular Breeding. 38(6):p. 80 [1-12]. |
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| DOI: 10.1007/s11032-018-0775-4 |
| Web URL(s):
https://link.springer.com/article/10.1007%2Fs11032-018-0775-4
Last checked: 07/20/2018
Access conditions: Item is within a limited-access website
https://link.springer.com/content/pdf/10.1007%2Fs11032-018-0775-4.pdf
Last checked: 07/20/2018
Requires: PDF Reader
Access conditions: Item is within a limited-access website |
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