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| Web URL(s): | https://scisoc.confex.com/scisoc/2018am/meetingapp.cgi/Paper/112563
Last checked: 11/14/2018
Requires: JavaScript |
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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Wang, Ruying;
Clarke, Bruce B.;
Belanger, Faith C. |
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| Author Affiliation: | Wang: Rutgers University, New Brunswick, NJ; Clarke: Department of Plant Biology, Rutgers University, New Brunswick, NJ; Belanger: Department of Plant Biology and Pathology, Rutgers University, New Brunswick, NJ |
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| Title: | Differential gene expression in the Epichloë festucae-Festuca rubra association during choke disease |
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| Section: | C05 turfgrass science
Other records with the "C05 turfgrass science" Section
Molecular techniques, genetics and plant breeding II: Abiotic and biotic stress oral (includes student competition)
Other records with the "Molecular techniques, genetics and plant breeding II: Abiotic and biotic stress oral (includes student competition)" Section
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| Meeting Info.: | Baltimore, Maryland: November 4-7, 2018 |
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| Source: | ASA, CSSA and SSSA International Annual Meetings. 2018, p. 112563. |
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| Publishing Information: | [Madison, Wisconsin]: [American Society of Agronomy, Crop Science Society of America, and Canadian Society of Agronomy] |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Choke disease; Epichloe festucae; Festuca rubra; Gene expression; Genetic analysis
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| Abstract/Contents: | "Epichlo ^De endophytes of cool-season grasses can benefit their hosts by protection from vertebrate and invertebrate pests, resistance to fungal diseases, and enhanced drought tolerance. Despite these benefits, some species can also be antagonistic to their hosts resulting in choke disease. During the host plant reproductive stage, the sexual fungal species can proliferate and form a stroma (choke) that arrests the development of the inflorescence. As a result, choke disease can significantly reduce seed yield. The objective of this study was to identify genes that were responsible for the dramatic change from restricted to proliferative fungal growth that results in choke disease. RNA-seq analysis was performed to compare gene expression of symptomatic and asymptomatic inflorescences of a field-grown strong creeping red fescue (Festuca rubra subsp. rubra). Three replicates of healthy developing inflorescences and choked panicles, respectively, were collected and RNA was extracted. Over 96 million reads per replicate per sample were generated by Illumina sequencing. Reads were first mapped to the reference genome E. festucae E2368. The plant assembly was generated by de novo assembling the unmapped reads. Differential gene expression analysis suggested that 1.65% (154) and 1.61% (150) of the endophyte genes were up- and down-regulated in the choke tissues, respectively; whereas, approximately 3.94% (2965) and 6.46% (4861) of the plant genes were up- and down-regulated in the choke tissues, respectively." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only!
"71-6" |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Wang, R., B. B. Clarke, and F. C. Belanger. 2018. Differential gene expression in the Epichloë festucae-Festuca rubra association during choke disease. Agron. Abr. p. 112563. |
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Last checked: 11/14/2018
Requires: JavaScript |
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