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| Web URL(s): | https://scisoc.confex.com/scisoc/2019am/meetingapp.cgi/Paper/119283
Last checked: 12/04/2019
Requires: JavaScript |
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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Shim, JungHyun;
Cushman, Kevin;
Karampudi, Naga Bhushana Rao;
Hinze, Lori L.;
Sweeney, Megan;
de Reyes, Benildo |
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| Author Affiliation: | Shim and de Reyes: Department of Plant Science and Soil Science, Texas Tech University, Lubbock, TX; Cushman: Texas Tech University, Lubbock, TX; Karampudi: PSS, Texas Tech University, Lubbock, TX; Hinze: USDA-ARS, College Station, TX; Sweeney: Agroscience BASF, North Carolina, NC |
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| Title: | Exploring novel genetic donors or salt tolerance across the tetraploid cultivated cotton germplasm (Gossypium hirsutum) by transcriptome profiling and genetic network modeling |
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| Section: | C05 turfgrass science
Other records with the "C05 turfgrass science" Section
Molecular techniques, genetics and plant breeding oral (includes student competition)
Other records with the "Molecular techniques, genetics and plant breeding oral (includes student competition)" Section
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| Meeting Info.: | San Antonio, Texas: November 10-13, 2019 |
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| Source: | ASA, CSSA and SSSA International Annual Meetings. 2019, p. 119283. |
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| Publishing Information: | [Madison, Wisconsin]: [American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America] |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Cultivar evaluation; Gene expression; Genetic analysis; Genetics model; Gossypium hirsutum; Ribonucleic acid; Salinity stress; Salt tolerance; Tetraploidy
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| Abstract/Contents: | "Exploring novel genetic donors of salt tolerance across the tetraploid cultivated cotton germplasm (Gossypium hirsutum) by transcriptome profiling and genetic network modeling. Junghyun Shim1, Kevin Cushman1, Naga Bhushana Rao Karampudi1, Lori Hinze2, Megan Sweeney3, Benildo G. de los Reyes1* 1Department of Plant and Soil Science, 219 Experimental Sciences Building, Texas Tech University, Lubbock, TX, 79409, USA. 2USDA-ARS, Crop Germplasm Research Unit, College Station, TX 77845, USA. 3BASF Corporation, Tech 3, 407 Davis Drive, Morrisville, NC 27560, United States. The paradigm for the identification of genes involved in abiotic stress tolerance mechanisms in plants has mostly been based on with differential expression. Such an approach has been fruitful in terms of revealing critical mechanisms that can be correlated with biochemical and physiological responses. In this study we performed a comparative analysis of the salt stress response transcriptomes of a minimal comparative panel representing an extreme contrast for salt tolerance across the Gossypium Diversity Reference Set. RNA-Seq libraries were constructed from the roots and shoots of highly salt-tolerant and highly salt-sensitive cultivars at 0h, 24h, 72h, 96h, 144h after mild to moderate levels of salt stress. From these datasets, we identified 1,179 differentially expressed genes (DEGs) with high stringency of over 2 folds difference, including 597 genes in the shoot and 582 genes in root. Among 1,179DEGs, a subset of 814 were upregulated in both tolerant and sensitive genotypes. Interestingly the expression increased exponentially across the entire duration of the stress experiment in the susceptible genotype, but only gradual increases were observed in the tolerant genotype with 2-fold to 200-fold difference between genotypes. Another subset of 123DEGs were upregulated in the sensitive genotype but downregulated in the tolerant genotype. On the other hand, the expression of a subset of 152DEGs increased exponentially in the tolerant genotype and gradually increased in the sensitive genotype and another subset of 42DEGs were upregulated in the tolerant genotype but downregulated in the sensitive genotype. Extreme upregulation of a subset of 937 genes were associated with susceptibility. Gene ontology analysis classified the 1179DEGs to 17 functional categories including 142 genes of transcription factors. We propose that under salinity stress, the susceptible genotype expends maximum effort to escape the abiotic challenge. This is expressed in terms of over expression of genes conferring tolerance." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only!
"218-1" |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Shim, J., K. Cushman, N. B. R. Karampudi, L. L. Hinze, M. Sweeney, and B. de Reyes. 2019. Exploring novel genetic donors or salt tolerance across the tetraploid cultivated cotton germplasm (Gossypium hirsutum) by transcriptome profiling and genetic network modeling. Agron. Abr. p. 119283. |
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Last checked: 12/04/2019
Requires: JavaScript |
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