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| DOI: | 10.1094/PDIS-02-21-0399-RE |
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| Web URL(s): | https://apsjournals.apsnet.org/doi/full/10.1094/PDIS-02-21-0399-RE
Last checked: 03/02/2022
Access conditions: Item is within a limited-access website
https://apsjournals.apsnet.org/doi/pdf/10.1094/PDIS-02-21-0399-RE
Last checked: 03/02/2022
Requires: PDF Reader
Access conditions: Item is within a limited-access website |
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| Publication Type:
| Refereed |
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| Author(s): | Rushford, Clayton A.;
North, Rebecca L.;
Miller, Gerald L. |
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| Author Affiliation: | Rushford and Miller: Division of Plant Science, University of Missouri, Columbia, MO; North: School of Natural Resources, University of Missouri, Columbia, MO |
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| Title: | Detection of Pythium spp. in golf course irrigation systems |
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| Section: | Research
Other records with the "Research" Section
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| Source: | Plant Disease. Vol. 106, No. 1, January 2022, p. 46-56. |
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| Publishing Information: | [Washington, D.C.]: Bureau of Plant Industry, U.S. Dept. of Agriculture |
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| # of Pages: | 11 |
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| Related Web URL: | https://apsjournals.apsnet.org/doi/abs/10.1094/PDIS-02-21-0399-RE
Last checked: 03/02/2022
Notes: Abstract only |
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| Keywords: | Author-Supplied Keywords: Oomycetes; Turf; Pathogen detection; Epidemiology; Disease development and spread
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| Abstract/Contents: | "Many Pythium spp. are causal agents of diseases of turfgrasses. Pythium spp. disseminate through irrigation systems in agricultural settings, and this study provides evidence that Pythium spp. also disseminate through golf course irrigation. Water samples were collected from irrigation heads and water sources at 10 golf courses in Missouri and Kansas, U.S.A. Samples were collected from 2018 to 2019 in April, July, and October. Phosphorus, nitrogen, and chloride concentrations were measured from irrigation head samples to determine if these parameters influence frequency of Pythium spp. detected. Pythium spp. were detected in samples through baiting and membrane filtration. Cultures were isolated on PARP media, and DNA was extracted from putative Pythium isolates. The internal transcribed spacer region was PCR-amplified and sequenced. Phylogenetic trees were constructed using representative sample sequences, sequences from seven morphologically identified reference isolates of Pythium, and similar GenBank accessions. Detected oomycete species include Lagenidium giganteum, Pythium biforme, Pythium insidiosum, Pythium marsipium, Pythium plurisporium, and Saprolegnia hypogyna. Twenty-one clades lacked species-level resolution, and 14 of these clades were associated with Pythium species. Clades A, C, D, E, I, and M contain Pythium species that cause root and crown rot on creeping bentgrass. Detected Pythium communities were dependent on the detection method used and sampling source. Pythium frequency and diversity were highest in April 2019. Sample temperature, sampling site, and chloride and nutrient concentrations did not influence Pythium frequency in samples. Irrigation systems using surface water sources contained at least three Pythium spp. over the course of 2 years." |
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| Language: | English |
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| References: | 58 |
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| Note: | Figures
Tables
Graphs |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Rushford, C. A., R. L. North, and G. L. Miller. 2022. Detection of Pythium spp. in golf course irrigation systems. Plant Disease. 106(1):p. 46-56. |
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| DOI: 10.1094/PDIS-02-21-0399-RE |
| Web URL(s):
https://apsjournals.apsnet.org/doi/full/10.1094/PDIS-02-21-0399-RE
Last checked: 03/02/2022
Access conditions: Item is within a limited-access website
https://apsjournals.apsnet.org/doi/pdf/10.1094/PDIS-02-21-0399-RE
Last checked: 03/02/2022
Requires: PDF Reader
Access conditions: Item is within a limited-access website |
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MSU catalog number: b2191617 |
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