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| DOI: | 10.1094/PDIS-04-20-0726-RE |
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| Web URL(s): | https://apsjournals.apsnet.org/doi/10.1094/PDIS-04-20-0726-RE
Last checked: 03/04/2022
Access conditions: Item is within a limited-access website
https://apsjournals.apsnet.org/doi/pdf/10.1094/PDIS-04-20-0726-RE
Last checked: 03/04/2022
Requires: PDF Reader
Access conditions: Item is within a limited-access website |
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| Publication Type:
| Refereed |
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| Author(s): | Groben, Glen;
Clarke, Bruce B.;
Murphy, James;
Koch, Paul;
Crouch, Jo Anne;
Lee, Sangkook;
Zhang, Ning |
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| Author Affiliation: | Groben, Clarke, and Murphy: Department of Plant Biology, Rutgers University, New Brunswick, NJ; Koch: Department of Plant Pathology, University of Wisconsin-Madison, Madison, WI; Crouch: Mycology and Nematology Genetic Diversity and Biology Laboratory, United States Department of Agriculture-Agricultural Research Service, Beltsville, MD; Lee: Department of Plant Biology, Rutgers University, New Brunswick, NJ and Department of Biotechnology, Hoseo University, Chungnam, South Korea; Zhang: Department of Plant Biology and Department of Biochemistry and Microbiology, Rutgers University, New Brunswick, NJ |
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| Title: | Real-time PCR detection of Clarireedia spp., the causal agents of dollar spot in turfgrasses |
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| Section: | Research
Other records with the "Research" Section
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| Source: | Plant Disease. Vol. 104, No. 12, December 2020, p. 3118-3123. |
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| Publishing Information: | [Washington, D.C.]: Bureau of Plant Industry, U.S. Dept. of Agriculture |
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| # of Pages: | 6 |
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| Related Web URL: | https://apsjournals.apsnet.org/doi/abs/10.1094/PDIS-04-20-0726-RE
Last checked: 03/04/2022
Notes: Abstract only |
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| Keywords: | Author-Supplied Keywords: Bentgrass; Pathogen detection; qPCR; Rutstroemiaceae
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| Abstract/Contents: | "Dollar spot is one of the most economically important diseases of turfgrasses. Recent taxonomic revisions have placed the dollar spot fungal pathogens in the new genus Clarireedia, with five species described. The main goal of this study was to develop a quantitative real-time PCR (qPCR) molecular detection assay based on the internal transcribed spacer (ITS) of the ribosomal RNA genes to quantify the abundance of Clarireedia spp. from environmental (field) samples. The qPCR assay was able to detect isolates of the four tested Clarireedia spp. but did not cross react with nontarget fungi, including closely related taxa, other turfgrass pathogens, or other fungal species commonly isolated from turfgrass. The assay is capable of detecting as little as 38.0 fg (3.8 × 10-14 g) of Clarireedia genomic DNA in 3 h. The qPCR assay detected Clarireedia spp. in both symptomatic and asymptomatic creeping bentgrass (Agrostis stolonifera) foliar tissue. Clarireedia spp. were rarely detected in the thatch or soil, indicating that these pathogens are not widely distributed in these areas of the environment. The fact that the pathogen was detected in asymptomatic tissue suggests that creeping bentgrass may be able to tolerate a certain quantity of the pathogens in leaves before disease symptoms appear; however, further research is needed to validate this hypothesis." |
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| Language: | English |
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| References: | 38 |
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| Note: | Pictures, color
Tables
Graphs |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Groben, G., B. B. Clarke, J. Murphy, P. Koch, J. A. Crouch, S. Lee, et al. 2020. Real-time PCR detection of Clarireedia spp., the causal agents of dollar spot in turfgrasses. Plant Disease. 104(12):p. 3118-3123. |
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| DOI: 10.1094/PDIS-04-20-0726-RE |
| Web URL(s):
https://apsjournals.apsnet.org/doi/10.1094/PDIS-04-20-0726-RE
Last checked: 03/04/2022
Access conditions: Item is within a limited-access website
https://apsjournals.apsnet.org/doi/pdf/10.1094/PDIS-04-20-0726-RE
Last checked: 03/04/2022
Requires: PDF Reader
Access conditions: Item is within a limited-access website |
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MSU catalog number: b2191617 |
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