Full TGIF Record # 317104
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Web URL(s):https://scisoc.confex.com/scisoc/2021am/meetingapp.cgi/Paper/133750
    Last checked: 03/30/2022
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Publication Type:
Content Type:Abstract or Summary only
Author(s):Tucker, Matthew; Eisenback, Jon D.; McCall, David S.
Author Affiliation:Tucker: Virginia Tech, Blacksburg, VA; Eisenback and McCall: School of Plant and Environmental Sciences, Virginia Tech, Blacksburg, VA
Title:Evaluating methods of plant parasitic nematode extraction from a golf course putting green
Section:C05 turfgrass science
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Turfgrass pest management poster: Diseases, insects, weeds (includes student competition)
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Meeting Info.:Salt Lake City, Utah: November 7-10, 2021
Source:ASA, CSSA and SSSA International Annual Meetings. 2021, p. 133750.
Publishing Information:[Madison, Wisconsin]: [American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America]
# of Pages:1
Abstract/Contents:"Golf course nematode samples are common in diagnostic labs and typically consist of multiple soil cores that remain intact to root depth. Cores typically remain intact during elutriation, potentially trapping plant-parasitic nematodes (PPN) within the root and soil matrix. The goal of this study was to determine if manipulating soil core integrity affects the extraction efficiency from golf course putting green samples. Soil cores were collected from a 007 bentgrass putting green in Blacksburg, VA at a depth of 15cm. Cores and soil material were mixed in a bucket to produce one homogenous soil sample where cores represent most of the variability. Subsamples were selected from the homogenized mix with each subsample containing five cores and soil totaling 250cc. The integrity of the soil cores in each subsample was altered by the following treatments: 1) cores left intact, 2) cut with scissors into 4-8 pieces (1cm), 3) shredded by hand into 4-8 pieces (1cm), and 4) blended for two minutes on high. A subsample from each treatment was randomly assigned and elutriated simultaneously in a four-bay semi-automatic elutriator. This was repeated five times for a total of 24 subsamples. Extracted nematodes were counted and identified to genus under magnification. Nematode counts were analyzed using JMP Pro 15 (Cary, NC) to determine differences in treatments. An ANOVA of means showed no significant differences in the total number of PPN extracted by treatment (p=0.726). There were also no significant differences of treatment by genus. These results indicate that manipulating soil core integrity does not affect extraction of PPN from golf course putting greens. Repetition of this study with samples from other locations, at differing turfgrass canopy health, from different turfgrass type and cultivar, and with different extraction methods is necessary to validate the implications of this study."
Note:"Poster # 1234"
Section "159"
This item is an abstract only!
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Tucker, M., J. D. Eisenback, and D. S. McCall. 2021. Evaluating methods of plant parasitic nematode extraction from a golf course putting green. Agron. Abr. p. 133750.
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    Last checked: 03/30/2022
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