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Web URL(s): | http://www.apsnet.org/publications/phytopathology/backissues/Documents/1994Articles/phyto84n10_1061.pdf#page=37 Last checked: 10/16/2015 Requires: PDF Reader Notes: Item is within a single large file |
Publication Type:
| Report |
Content Type: | Abstract or Summary only |
Author(s): | Bunting, T. E.;
Plumley, K. A.;
Clarke, B. B.;
Hillman, B. I. |
Author Affiliation: | Department of Plant Pathology, Rutgers University, New Brunswick, NJ |
Title: | Phylogenic comparison of Magnaporthe spp. using ITS-1 sequence analysis |
Meeting Info.: | 1994 APS Annual Meeting, August 6-10, 1994, Albuquerque, New Mexico |
Source: | Phytopathology. Vol. 84, No. 10, October 1994, p. 1097. |
Publishing Information: | St. Paul, MN: American Phytopathological Society |
# of Pages: | 1 |
Keywords: | TIC Keywords: Magnaporthe poae; Disease identification; DNA; Poa; Fine fescues; Phylogeny
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Abstract/Contents: | "Magnaporthe poae causes summer patch disease of bluegrasses and fine fescues. The fungus is a heterothallic ascomycete that does not readily sporulate in culture and has not been found to sporulate in nature. We have designed primers which amplify a 453 base pair product from M. poae DNA and may be used for rapid identification. A closely related homothallic fungus, M. rhizophila will also amplify a similar-sized product with the primers. Sequences from the first internal transcribed spacer region (ITS-1) of the nuclear ribosomal DNA from Magnaporthe spp., Gaeumannomyces spp., and Colletorichium (Glomerella) sp. were analyzed using parsimony analysis. Interestingly M. rhizophila and the M. poae isolates all clustered out from the others with an 88% bootstrap frequency. The other fungi did not resolve except for two isolates of Gaeumannomyces graminis var. graminis (83% bootstrap frequency). We are currently studying enzyme activities of M. poae that may be important for virulence such as [Beta]-fructofuranosidase and lignin peroxidases." |
Language: | English |
References: | 0 |
Note: | This item is an abstract only! |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Bunting, T. E., K. A. Plumley, B. B. Clarke, and B. I. Hillman. 1994. Phylogenic comparison of Magnaporthe spp. using ITS-1 sequence analysis. Phytopathology. 84(10):p. 1097. |
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| Web URL(s): http://www.apsnet.org/publications/phytopathology/backissues/Documents/1994Articles/phyto84n10_1061.pdf#page=37 Last checked: 10/16/2015 Requires: PDF Reader Notes: Item is within a single large file |
| MSU catalog number: SB 599 .P48 |
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