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| Web URL(s): | https://scisoc.confex.com/scisoc/2022am/meetingapp.cgi/Paper/143460
Last checked: 01/24/2023
Requires: JavaScript; HTML5 |
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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Dhillon, Kamalpreet Kaur;
Rueda-Kunz, Dario D.;
Chavez, Dario J.;
Czarnota, Mark;
Itle, Rachel A.;
Schwartz, Brian M. |
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| Author Affiliation: | Dhillon: Presenting Author and University of Georgia; Rueda-Kunz, Chavez, Czarnota, and Itle: University of Georgia; Schwartz: University of Georgia-Tifton |
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| Title: | Pollen microscopy in zoysiagrass advanced breeding selection lines for use in haploid production through anther culture |
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| Section: | Turfgrass Physiology, Molecular Biology, Microbiome, and Genetics Poster (includes student competition)
Other records with the "Turfgrass Physiology, Molecular Biology, Microbiome, and Genetics Poster (includes student competition)" Section
C05 turfgrass science
Other records with the "C05 turfgrass science" Section
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| Meeting Info.: | Baltimore, Maryland: November 6-9, 2022 |
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| Source: | ASA, CSSA, SSSA International Annual Meeting. 2022, p. 143460. |
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| Publishing Information: | [Madison, Wisconsin]: [American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America] |
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| # of Pages: | 1 |
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| Abstract/Contents: | "Zoysiagrasses (Zoysia spp. Willd.) are allotetraploid (2n=4x=40) perennial warm season grasses. They are tolerant to drought, low fertility, salinity and requires low maintenance. One major limitation is seed head production when irregularly mowed, leading to genetic variability, non-uniformity and reduced quality. Currently, ploidy variation to aid breeding of sterile (triploid) cultivars does not exist, and development of diploid germplasm would be beneficial. The objective of this study was to examine pollen mother cells (PMCs) in tetraploid germplasm for haploid induction through anther culture. Five breeding selection were grown in the greenhouse in 2018. Flowers were collected during fall season, classified into five developmental stages [1-5(mature)], and three flowers (replicates)/stage totaling 15 flowers. Flowers were divided into three subsections/stage [top (T), middle (M), and bottom(B)], fixed with Formaldehyde Alcohol Acetic Acid, stained with 1% acetocarmine, and evaluated under microscope at 400x. Ten PMCs were scored for stages in meiosis I & II and mitosis within each subsection, totaling 450 PMCs. Pollen mitosis stages of mid-uninucleate to late uninucleate were targeted based on haploid induction success in other crops. Overall, PMCs within these stages were concentrated in 3T (100% 09-TZ-53-20 and 16-TZ-12143, 33.3% 29-2B7 and 15-TZ-10696, 0% L1F), 4M (100% 09-TZ-53-20, 66.67% 16-TZ-12143 and 29-2B7, 0% 15-TZ-10696 and L1F), and 4T (100% 09-TZ-53-20, 16-TZ-12143 and 29-2B7, 33.3% L1F, 0% 15-TZ-10696). Results suggest that stages 3T, 4M and 4T are most suitable for anther culture and will be used as a starting point for the creation of novel sterile triploid zoysiagrass cultivars." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only!
"372" |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Dhillon, K. K., D. D. Rueda-Kunz, D. J. Chavez, M. Czarnota, R. A. Itle, and B. M. Schwartz. 2022. Pollen microscopy in zoysiagrass advanced breeding selection lines for use in haploid production through anther culture. Agron. Abr. p. 143460. |
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| Web URL(s):
https://scisoc.confex.com/scisoc/2022am/meetingapp.cgi/Paper/143460
Last checked: 01/24/2023
Requires: JavaScript; HTML5 |
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