Full TGIF Record # 324931
Item 1 of 1
Web URL(s):https://scisoc.confex.com/scisoc/2022am/meetingapp.cgi/Paper/143460
    Last checked: 01/24/2023
    Requires: JavaScript; HTML5
Publication Type:
Content Type:Abstract or Summary only
Author(s):Dhillon, Kamalpreet Kaur; Rueda-Kunz, Dario D.; Chavez, Dario J.; Czarnota, Mark; Itle, Rachel A.; Schwartz, Brian M.
Author Affiliation:Dhillon: Presenting Author and University of Georgia; Rueda-Kunz, Chavez, Czarnota, and Itle: University of Georgia; Schwartz: University of Georgia-Tifton
Title:Pollen microscopy in zoysiagrass advanced breeding selection lines for use in haploid production through anther culture
Section:Turfgrass Physiology, Molecular Biology, Microbiome, and Genetics Poster (includes student competition)
Other records with the "Turfgrass Physiology, Molecular Biology, Microbiome, and Genetics Poster (includes student competition)" Section

C05 turfgrass science
Other records with the "C05 turfgrass science" Section
Meeting Info.:Baltimore, Maryland: November 6-9, 2022
Source:ASA, CSSA, SSSA International Annual Meeting. 2022, p. 143460.
Publishing Information:[Madison, Wisconsin]: [American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America]
# of Pages:1
Abstract/Contents:"Zoysiagrasses (Zoysia spp. Willd.) are allotetraploid (2n=4x=40) perennial warm season grasses. They are tolerant to drought, low fertility, salinity and requires low maintenance. One major limitation is seed head production when irregularly mowed, leading to genetic variability, non-uniformity and reduced quality. Currently, ploidy variation to aid breeding of sterile (triploid) cultivars does not exist, and development of diploid germplasm would be beneficial. The objective of this study was to examine pollen mother cells (PMCs) in tetraploid germplasm for haploid induction through anther culture. Five breeding selection were grown in the greenhouse in 2018. Flowers were collected during fall season, classified into five developmental stages [1-5(mature)], and three flowers (replicates)/stage totaling 15 flowers. Flowers were divided into three subsections/stage [top (T), middle (M), and bottom(B)], fixed with Formaldehyde Alcohol Acetic Acid, stained with 1% acetocarmine, and evaluated under microscope at 400x. Ten PMCs were scored for stages in meiosis I & II and mitosis within each subsection, totaling 450 PMCs. Pollen mitosis stages of mid-uninucleate to late uninucleate were targeted based on haploid induction success in other crops. Overall, PMCs within these stages were concentrated in 3T (100% 09-TZ-53-20 and 16-TZ-12143, 33.3% 29-2B7 and 15-TZ-10696, 0% L1F), 4M (100% 09-TZ-53-20, 66.67% 16-TZ-12143 and 29-2B7, 0% 15-TZ-10696 and L1F), and 4T (100% 09-TZ-53-20, 16-TZ-12143 and 29-2B7, 33.3% L1F, 0% 15-TZ-10696). Results suggest that stages 3T, 4M and 4T are most suitable for anther culture and will be used as a starting point for the creation of novel sterile triploid zoysiagrass cultivars."
Note:This item is an abstract only!
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Dhillon, K. K., D. D. Rueda-Kunz, D. J. Chavez, M. Czarnota, R. A. Itle, and B. M. Schwartz. 2022. Pollen microscopy in zoysiagrass advanced breeding selection lines for use in haploid production through anther culture. Agron. Abr. p. 143460.
Fastlink to access this record outside TGIF: https://tic.msu.edu/tgif/flink?recno=324931
If there are problems with this record, send us feedback about record 324931.
Choices for finding the above item:
Web URL(s):
    Last checked: 01/24/2023
    Requires: JavaScript; HTML5
Request through your local library's inter-library loan service (bring or send a copy of this TGIF record)