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| Publication Type:
| Refereed |
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| Author(s): | Booy, G.;
van Dreven, F.;
Steverink-Raben, A. |
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| Author Affiliation: | Centre for Plant Breeding and Reproduction Research CPRO-DLO Wageningen, The Netherlands |
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| Title: | Identification of ryegrass varieties ( olium spp.) using allele frequencies of the PGI-2 and ACP-1 isozyme systems |
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| Source: | Plant Varieties and Seeds. Vol. 6, No. 3, December 1993, p. 179-195. |
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| Publishing Information: | Oxford: Blackwell Scientific Publications |
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| # of Pages: | 17 |
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| Keywords: | TIC Keywords: Lolium; Lolium perenne; Lolium multiflorum; Varieties; Alleles; Isoenzymes; Acid phosphatase; Phosphoglucoisomerase; SDS-PAGE; Diploids
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| Abstract/Contents: | "A collection of 431 diploid and tetraploid varieties of ryegrass, belonging to the cross-fertilizing species Lolium perenne, L. multiflorum, or their interspecific hybrid L. x boucheanum were analysed for their PGI-2 (phosphoglucoisomerase), and 312 varieties for their ACP-1 (acid phosphatase) allele frequencies by polyacrylamide gel electrophoresis (PAGE). PGI-2 allele frequencies were collected from 161 varieties from other countries and 88 of these were also analysed in this study. All diploids were tested for Hardy Weinberg (HW) equilibrium: 99% appeared to be in HW equilibrium for PGI-2 and 85% for ACP-1 (P=0.01). Dendrograms were constructed of those diploids which were in HW equilibrium and of all tetraploids analysed. On the basis of these dendrograms varieties were classified into groups. For PGI-2, the 355 diploids could be classified into 23 groups and the 145 tetraploids into 31 groups. For ACP-1 the 160 diploids could be classified into 18 groups, the 85 tetraploids into 17 groups. On the basis of a combination of PGI-2 and ACP-1 allele frequencies, 40 groups could be discriminated for the 160 diploids and 30 groups for the 85 tetraploids. In 89 of the 312 varieties analysed for ACP-1 (29%), one or more plants with an aberrant banding pattern were observed; in 36 of these 89 varieties (40%), a zymogram was found which was homozygous for a 'silent allele'. The species L. perenne and L. multiflorum could be discriminated on the basis of differences in PGI-2 frequencies. Using ACP-1, it was not possible to discriminate between the two species. For 98 varieties of the 125 (78%), from which PGI-2 allele frequencies from two or more countries were available, no significant differences between countries were observed (P=0.05). Possible applications of isozyme allele frequencies for variety identification and registration for plant breeders' rights are discussed." |
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| Language: | English |
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| References: | 23 |
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| Note: | Figures
Tables |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Booy, G., F. van Dreven, and A. Steverink-Raben. 1993. Identification of ryegrass varieties ( olium spp.) using allele frequencies of the PGI-2 and ACP-1 isozyme systems. Plant Var. Seeds. 6(3):p. 179-195. |
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