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| DOI: | 10.11686/cyxb2022276 |
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| Web URL(s): | http://cyxb.magtech.com.cn/CN/10.11686/cyxb2022276
Last checked: 06/16/2023
Access conditions: Item is within a limited-access website |
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| Publication Type:
| Report |
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| Author(s): | Cui, Ting;
Wang, Yong;
Ma, Hui-ling |
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| Author Affiliation: | College of Pratacultural Science, Gansu Agricultural University, Key Laboratory of Grassland Ecosystem, Ministry of Education, Pratacultural Enginnering Laboratory of Gansu Providence, Sino-U.S. Center for Grazing Land Ecosystem Sustainability, Lanzhou, China |
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| Title: | Analysis of the key exogenous IAA-induced gene expression levels and metabolic pathways involved in long-distance translocation of Cd in Poa pratensis |
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| Source: | [Caoye Xuebao] [Acta Prataculturae Sinica]. Vol. 32, No. 6, June 2023, p. 146-156. |
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| Publishing Information: | [Lanzhou Shi, China]: ["Cao Ye Xue Bao" Bian Ji Wei Yuan Hui] |
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| # of Pages: | 11 |
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| Keywords: | Author-Supplied Keywords: Cd stress; Kentucky bluegrass (Poa Pratensis); Auxin transcriptome; Translocation of Cd
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| Abstract/Contents: | "This research aimed to investigate the effects and molecular mechanisms of indole-3-acetic acid (IAA)-induced decrease of the long-distance translocation of cadmium (Cd) from the roots to the shoots in Kentucky bluegrass by analysis the differentially expressed IAA-induced genes (DEGs) and metabolic pathways involved in response to Cd stress. The species Poa pratensis was used as the study material and 400 nmol·L-1 IAA was sprayed on the leaves of plants subjected to 600 µmol·L-1 of Cd stress. Plant heights, root lengths, and Cd concentrations in the roots and leaves were measured, and transcriptome sequencing was performed in both the Cd+ IAA and Cd treatments. Real-time quantitative polymerase chain reaction (RT-qPCR) was used to verify the accuracy of the RNA-seq. It was found that exogenous IAA significantly increased the plant heights and root lengths, and inhibited the long-distance translocation of Cd from roots to leaves. There were 1294 genes with significantly IAA-modified expression under Cd stress in Kentucky bluegrass. The expression levels of RT-qPCR had a high correlation with RNA-seq data. Gene ontology (GO) enrichment analysis showed that the genes involved in response to heat, cold, Cd, and virus exposure were induced by IAA. The molecular chaperone HSP70, protein kinase mitogen-activated protein kinase (MAPK), and transcription factor myeloblastosis46 (MYB46) were very obviously up-regulated by IAA application. In addition, genes involved in the glutathione metabolism and lignin biosynthesis pathways were also up-regulated by exogenous IAA. This may be indicative of increased glutathione synthesis and lignification of the cell wall and further increase the vacuolar compartmentalization and decreased the Cd entry into cells, and further decrease the Cd concentration in leaves. This study provides reference data on mechanisms of IAA-induced translocation and distribution of Cd." |
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| Language: | Chinese |
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| References: | 36 |
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| Note: | Abstract only
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Cui, T., Y. Wang, and H.-l. Ma. 2023. Analysis of the key exogenous IAA-induced gene expression levels and metabolic pathways involved in long-distance translocation of Cd in Poa pratensis. (In Chinese) [Caoye Xuebao] [Acta Prataculturae Sinica]. 32(6):p. 146-156. |
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| DOI: 10.11686/cyxb2022276 |
| Web URL(s):
http://cyxb.magtech.com.cn/CN/10.11686/cyxb2022276
Last checked: 06/16/2023
Access conditions: Item is within a limited-access website |
| 
MSU catalog number: b10289426 |
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