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| DOI: | 10.11686/cyxb2021382 |
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| Web URL(s): | http://cyxb.magtech.com.cn/CN/10.11686/cyxb2021382#1
Last checked: 06/19/2023
Notes: Abstract only |
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| Publication Type:
| Report |
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| Author(s): | Yao, Jia-ming;
He, Yue;
Hao, Huan-huan;
Huang, Xin-ru;
Zhang, Jing;
Xu, Bin |
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| Author Affiliation: | College of Agro-Grassland, Nanjing Agricultural University, Nanjing, China |
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| Title: | Characterization and transcriptional regulation analysis of the LpPIL5 gene in perennial ryegrass |
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| Source: | [Caoye Xuebao] [Acta Prataculturae Sinica]. Vol. 31, No. 9, September 2022, p. 155-167. |
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| Publishing Information: | [Lanzhou Shi, China]: ["Cao Ye Xue Bao" Bian Ji Wei Yuan Hui] |
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| # of Pages: | 13 |
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| Keywords: | Author-Supplied Keywords: Perennial ryegrass; Basic helix-loop-helix; Phytochrome-interacting factors; Subcellular localization; Transcription analysis
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| Abstract/Contents: | "Phytochrome-interacting factors (PIFs), a subfamily of the bHLH family, play important roles in plant light and hormone signaling, and stress tolerance. In the current study, a PIF gene was cloned from perennial ryegrass (Lolium perenne), and its genetic sequence was most closely related to Arabidopsis thaliana AtPIF5, therefore, it was named LpPIL5-like (LpPIL5). The coding region sequence (CDS) of this LpPIL5 gene is 1410 bp, containing 5 exons, encoding 470 amino acids. The protein it codes for has a typical bHLH domain and APB motif and is localized in the nucleus. The promoter region (1500 bp) of LpPIL5 has various cis-elements, including ABRE, MBS, G-box, and others, related to light, hormone, and stress responses. The results of transcription analysis showed that relative expression of LpPIL5 was high in leaves, but low in roots, stems, and leaf sheaths. The transcription of LpPIL5 was also regulated by circadian rhythm, and was significantly higher in light than in dark. In addition, its relative expression level in leaves was significantly inhibited under polyethylene glycol (PEG), NaCl, CdCl2, high temperature stresses, and by N-(phenylmethyl)-9H-purin-6-amine (6-BA) and abscisic acid (ABA) treatments. In roots, the relative expression of the LpPIL5 gene increased significantly after 12 h of these treatments, and became significantly higher than in control plants after 24 h of treatment." |
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| Language: | Chinese |
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| References: | 44 |
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| Note: | Abstract also appears in English
Pictures, color
Figures
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Yao, J.-m., Y. He, H.-h. Hao, X.-r. Huang, J. Zhang, and B. Xu. 2022. Characterization and transcriptional regulation analysis of the LpPIL5 gene in perennial ryegrass. (In Chinese) [Caoye Xuebao] [Acta Prataculturae Sinica]. 31(9):p. 155-167. |
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| DOI: 10.11686/cyxb2021382 |
| Web URL(s):
http://cyxb.magtech.com.cn/CN/10.11686/cyxb2021382#1
Last checked: 06/19/2023
Notes: Abstract only |
| 
MSU catalog number: b10289426 |
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