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| DOI: | 10.13817/j.cnki.cyycp.2022.03.002 |
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| Web URL(s): | http://cyycp.ijournals.cn/cyycp/article/abstract/20220302?st=article_issue
Last checked: 10/6/2023
Notes: Abstract only
http://cyycp.ijournals.cn/cyycp/article/pdf/20220302
Last checked: 01/17/2024
Requires: PDF Reader
Notes: Direct download |
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| Publication Type:
| Report |
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| Author(s): | Yuan, Hong;
Wang, Yu-yue;
Guo, Ai-qiang;
Liu, Ling-yun;
Fan, Xi-feng;
Wu, Ju-ying |
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| Author Affiliation: | Hong, Yu-yue, and Ai-qiang: Tianjin Binhai International Airport Co. Ltd, Tianjin, China; Ling-yun, Xi-feng and Ju-ying: Beijing Academy of Agriculture and Forestry Sciences, Beijing, China |
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| Title: | Optimization of ISSR molecular marker system and genetic diversity analysis of Buchloe dactyloides |
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| Source: | [Caoyuan yu Caoping] [Grassland and Turf]. Vol. 42, No. 3, 2022, p. 9-15. |
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| Publishing Information: | Lanzhou, China: Zhongguo cao yuan xue hui |
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| # of Pages: | 7 |
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| Keywords: | Author-Supplied Keywords: Buchloe dactyloides; ISSR molecular markers; System optimization; Cluster analysis; Genetic diversity
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| Abstract/Contents: | "In this study the optimal parameter of each factor in ISSR molecular marker system of buffalograss (Buchloe dactyloides) was explored based on L9(34) orthogonal test. Factors including Taq PCR Mix, primers, the amount of primer and template DNA, primer annealing temperature were examined. The optimal 10 µL system was 5.0 µL Mix, 40 ng DNA template, 0.6 µL primer (1. 0 µmol/L) and ddH2O complement. The best amplification condition was pre-denaturation for 3 min at 94°C, 34 cycles of denaturation at 94°C for 30 s, annealing at 48°C for 30 s, and extension at 72°C for 10min, followed by extension at 72°C for 10 min and stored at 4°C. The results of genetic diversity of 39 collected samples showed that all of them were divided into two groups by UPGMA cluster, among which No. 21 had the closest genetic relationship with the target female B. dactyloides of Tianjin Binhai International Airport. The germplasm No. 21 could be used as the basis for subsequent propagation. In this study, ISSR system exploration and optimization and genetic diversity analysis provided a basis for the genetic diversity analysis of buffalograss." |
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| Language: | Chinese |
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| References: | 29 |
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| Note: | Abstract also appears in English
Figures
Tables |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Yuan, H., Y.-y. Wang, A.-q. Guo, L.-y. Liu, X.-f. Fan, and J.-y. Wu. 2022. Optimization of ISSR molecular marker system and genetic diversity analysis of Buchloe dactyloides. (In Chinese) [Caoyuan yu Caoping] [Grassland and Turf]. 42(3):p. 9-15. |
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| DOI: 10.13817/j.cnki.cyycp.2022.03.002 |
| Web URL(s):
http://cyycp.ijournals.cn/cyycp/article/abstract/20220302?st=article_issue
Last checked: 10/6/2023
Notes: Abstract only
http://cyycp.ijournals.cn/cyycp/article/pdf/20220302
Last checked: 01/17/2024
Requires: PDF Reader
Notes: Direct download |
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