Full TGIF Record # 333404
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Web URL(s):https://scisoc.confex.com/scisoc/2023am/meetingapp.cgi/Paper/150587
    Last checked: 12/01/2023
Publication Type:
Content Type:Abstract or Summary only
Author(s):Cors, Jonathan
Author Affiliation:Presenting Author and Applied Plant Sciences, University of Minnesota - Twin Cities, Minneapolis, MN
Title:Progressing towards biocontainment in perennial ryegrass with improved transformation protocols
Section:Turfgrass breeding, genetics, molecular biology, microbiome oral I (includes student competition)
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C05 turfgrass science
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Meeting Info.:St. Louis, Missouri: October 29-November 1, 2023
Source:ASA, CSSA, SSSA International Annual Meeting. 2023, p. 150587.
Publishing Information:[Madison, Wisconsin]: [American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America]
# of Pages:1
Abstract/Contents:"A new biocontainment method named Engineered Genetic Incompatibility (EGI) has been proven to work in both yeast and Drosophila melanogaster. It utilizes CRISPR activators to overexpress integral genes leading to plant death as a result. As such, the only necessity for EGI to work within a species is the ability for CRISPR activators to work as well as having genes that when overexpressed can cause death. This biocontainment method has great potential to work in turfgrass, allowing for the further introgression of other beneficial genes. This talk will cover several experiments progressing towards this goal of EGI in turfgrass that have been conducted in the turfgrass species perennial ryegrass.Largely it will cover several aspects of perennial ryegrass transformation protocols have been tested and optimized, such as improvements in published callus culture protocols and testing of new protocols utilizing embryonic genes such as Wus and Bbm to produce embryonic callus. While these experiments have been conducted with EGI in mind, all of them can be utilized for general turfgrass transformation."
This item is an abstract only!
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Cors, J. 2023. Progressing towards biocontainment in perennial ryegrass with improved transformation protocols. Agron. Abr. p. 150587.
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    Last checked: 12/01/2023
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