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| Web URL(s): | https://scisoc.confex.com/scisoc/2023am/meetingapp.cgi/Paper/150375
Last checked: 12/01/2023 |
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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Yu, Xingwang;
Gouveia, Beatriz T.;
Van Der Laat, Rocio;
Carbajal Melgar, Esdras Manuel;
Milla-Lewis, Susana R. |
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| Author Affiliation: | Yu: Presenting Author and Department of Crop and Soil Sciences, North Carolina State University, Raleigh, NC; Gouveia: Biology, North Carolina State University, Raleigh, NC; Van Der Laat and Milla-Lewis: Department of Crop and Soil Sciences, North Carolina State University, Raleigh, NC; Carbajal Melgar: North Carolina, North Carolina State University, Raleigh, NC |
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| Title: | Validation of diagnostic molecular markers for marker-assisted breeding of gray leaf spot resistance in St. Augustinegrass |
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| Section: | Turfgrass physiology, molecular biology, breeding, genetic and microbiome poster (includes student competition)
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| Meeting Info.: | St. Louis, Missouri: October 29-November 1, 2023 |
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| Source: | ASA, CSSA, SSSA International Annual Meeting. 2023, p. 150375. |
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| Publishing Information: | [Madison, Wisconsin]: [American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America] |
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| # of Pages: | 1 |
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| Abstract/Contents: | "Gray leaf spot (GLS), caused by Magnaporthe grisea, is a major fungal disease of St. Augustinegrass [Stenotaphrum secundatum (Walt.) Kuntze], causing widespread blighting of the foliage under warm, humid conditions. To elucidate the genetic control of GLS resistance, we previously identified a total of 30 potential quantitative trait loci (QTL) associated with the trait. Among them, several regions of overlap were found on linkage groups 2, 5, 7, 8, 9, which attract interest because they might harbor real genetic loci. However, marker-assisted breeding for GLS has not been well established. To validate single nucleotide polymorphism (SNP) markers associated with GLS resistance, 20 markers in above QTL regions were selected to genotyping the St. Augustinegrass advanced breeding population. Based on evaluation of locus-specificity, call rate and clarity, 3 of them (GLS2, 19, 20) were successfully converted to PCR Allele Competitive Extension (PACE) markers. Levels of GLS resistance of St. Augustinegrass breeding population showed significant variation. Allelic substitution effects analysis showed that lines with desirable alleles (homozygous or heterozygous) showed lower GLS incidence for all three markers, while only GLS20 displayed an additive effect that double desirable alleles showed lower GLS incidence than heterozygous. These markers will facilitate marker-assisted breeding for gray leaf spot resistance in St. Augustinegrass." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Yu, X., B. T. Gouveia, R. Van der Laat, E. M. Carbajal Melgar, and S. R. Milla-Lewis. 2023. Validation of diagnostic molecular markers for marker-assisted breeding of gray leaf spot resistance in St. Augustinegrass. Agron. Abr. p. 150375. |
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| Web URL(s):
https://scisoc.confex.com/scisoc/2023am/meetingapp.cgi/Paper/150375
Last checked: 12/01/2023 |
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