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| Web URL(s): | https://conference.ifas.ufl.edu/iftbc2019/documents/IFTBC-2019-Online-Abstract-Book.pdf#page=95
Last checked: 04/04/2024
Requires: PDF Reader
Notes: Item is witihin a single large file |
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| Publication Type:
| Report |
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| Author(s): | Quesenberry, K. H. (Kenneth);
Kenworthy, K. E. (KEvin);
Buhlman, J.;
Cox, K.;
Carter, Z. |
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| Author Affiliation: | Quesenberry: Professor Emeritus and UF/IFAS Agronomy and Forage and Turf Breeding and Agronomy Department, University of Florida, Gainesville, FL; Kenworthy: Professor and UF/IFAS Agronomy and Turfgrass and Forage Breeding and Genetics and Agronomy Department, University of Florida, Gainesville, FL; Buhlman, Cox and Carter: Agronomy Department, University of Florida, Gainesville, FL |
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| Title: | Naturally occurring stable 8X and chimerical 4X/8X zoysiagrass genotypes revealed by flow cytometry |
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| Meeting Info.: | Lake Buena Vista, Florida: March 24-27, 2019 |
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| Source: | International Forage & Turf Breeding Conference. 2019, p. 95. |
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| Publishing Information: | Gainesville, Florida: UF/IFAS Office of Conferences & Institutes |
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| # of Pages: | 1 |
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| Abstract/Contents: | "Zoysiagrass (Zoysia Willd.) is increasingly selected for use as a turfgrass in warmer climatic zones. The Zoysia spp. are tetraploids, although recently both induced and naturally occurring octaploids were reported. Variable ploidy levels in a turfgrass can have advantages, e.g. sterile hybrids between ploidy levels and changes in morphological texture; or disadvantages, e.g. crossing barriers and genetic instability. Flow cytometry has been widely used in forage and turfgrass breeding programs as an aid in determining ploidy of naturally occurring and induced ploidy changes. This research reports evaluation of ploidy levels and morphology of naturally occurring variants of 'Empire' zoysiagrass and progeny from a naturally occurring octaploid line from Empire. The ploidy level and internal chimerical ploidy variability of a naturally occurring octaploid Empire zoysiagrass clone, other clones growing in the same field block, and certified Empire were evaluated by flow cytometry. Flow cytometry measurements were determined on pots established from single node cuttings from plugs taken from the field block. Known standards of sorghum or chicken erythrocytes were used to calculated 2c DNA values. Morphological differences in raceme lengths, seed head heights, blade lengths, and blade widths among these clones and a certified Empire clone were also determined. Empire Clone "E11" showed a consistent G1 peak double the value of certified Empire. Both of these clones had a "normal" G2 peak that consistently had 20% or fewer events than the "G1 peak", as usually seen with healthy growing vegetative tissue. In contrast, several clones sampled across the field block showed a "G1 peak" at the value equivalent to Empire and a second "G1 peak" equivalent to E11, with both peaks having similar cell event counts. These flow cytometry results remained consistent through frequent removal of vegetative growth (e.g. mowing) for more than one year. These results strongly suggest the presence of a periclinal L1/L2 chimera in some of the sampled clones. Research is underway to evaluate seedling progeny from the "chimerical clones" and the E11 clone to determine ploidy stability through a sexual seedling generation. Preliminary evaluations of ploidy of seedling progeny from open pollination of E11 are in progress. Morphological differences were identified between certified Empire and the E11 clone. Seed head height, raceme length, blade length and blade width were larger with the octaploid E11 clone compared to Empire. These morphological differences resulting from increased ploidy are consistent with other reported effects of chromosome doubling. The cause of these changes in ploidy are unknown. It has been speculated that the dinitroaniline herbicides (commonly used in turfgrasses) and also known to be a mitotic poison might be the source. Further research is needed to investigate the type and stability of these apparent chimerical zoysiagrass clones. Breeding behavior of these clones also needs to be investigated. The potential to use the octaploid E11 clone in crosses with tetraploid zoysiagrasses to produce sterile hexaploids is intriguing." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Quesenberry, K. H., K. E. Kenworthy, J. Buhlman, K. Cox, and Z. Carter. 2019. Naturally occurring stable 8X and chimerical 4X/8X zoysiagrass genotypes revealed by flow cytometry. International Forage & Turf Breeding Conference. p. 95. |
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| Web URL(s):
https://conference.ifas.ufl.edu/iftbc2019/documents/IFTBC-2019-Online-Abstract-Book.pdf#page=95
Last checked: 04/04/2024
Requires: PDF Reader
Notes: Item is witihin a single large file |
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