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| Web URL(s): | http://apsjournals.apsnet.org/doi/pdfplus/10.1094/PHYTO.1997.87.6.614
Last checked: 08/26/2010
Requires: PDF Reader |
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| Publication Type:
| Refereed |
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| Author(s): | Rodriguez, Fanny;
Pfender, William F. |
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| Author Affiliation: | Department of Plant Pathology, Throckmorton Hall, Kansas State University, Manhattan 66506-5502, USDA-ARS NFTSRC, 3450 SW Campus Way, Corvallis, OR 97331 |
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| Title: | Antibiosis and antagonism of Sclerotinia homeocarpa and Drechslera poae by Pseudomonas fluorescens Pf-5 in vitro and in planta |
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| Section: | Biological Control
Other records with the "Biological Control" Section
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| Source: | Phytopathology. Vol. 87, No. 6, June 1997, p. 614-621. |
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| Publishing Information: | St. Paul, MN: American Phytopathological Society |
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| # of Pages: | 8 |
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| Keywords: | TIC Keywords: Pseudomonas; Antibiotics; Disease control; Melting out; Dollar spot; Antagonism; Sclerotinia homoeocarpa; Drechslera poae; In vitro; Metabolites
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| Abstract/Contents: | "Pseudomonas fluorescens strain Pf-5, which produces several antifungal metabolites, including the antibiotics pyoluteorin, pyrrolnitrin, and 2,4-diacetylphloroglucinol, was tested for its ability to inhibit Sclerotinia homeocarpa (causal agent of dollar spot) and Drechslera poae (causal agent of 'melting-out') in vitro and in turfgrass; Tn5 mutants with altered antibiotic production also were tested. Inhibition in vitro differed with the medium used, but both fungi generaly were inhibited by Pf-5. In most cases, a mutant deficiet in pyoluteorin but not pyrrolnitrin or 2,4-diacetylphloroglucinol was as inhibitory as Pf-5, whereas a pyrrolnitrin-deficient mutant was less inhibitory than Pf-5 in most fungus/medium combinations. High-performance liquid chromatography analysis of culture extracts showed that bacterial genotype and nutrition have an interactive effect on antibiotic production, such that conditions causing an increase in one antibiotic may increase or decrease another. The purported deficiencies for the pyrrolnitrin- and pyoluteorin-deficient mutants were confirmed. In S. homoeocarpa-infested grass clippings incubated in a moist chamber, Pf-5 reduced mycelial growth, whereas the pyrrolnitrin-deficient mutant did not and the pyoluteorin-deficient mutant was intermediate. In greenhouse experiments, Pf-5 reduced dollar spot disease incidence in bentgrass and bluegrass when sprayed over inoculated turf. In grass clippings infested with D. poae and incubated in a moist chamber under favorable conditions for spore production, Pf-5 did not reduce significantly the number of spores produced compared with the nontreated control. However, Pf-5 reduced melting-out disease incidence and severity in bluegrass inoculated with spores of D. poae under greenhouse conditions." |
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| Language: | English |
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| References: | 32 |
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| Note: | Graphs
Tables |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Rodriguez, F., and W. F. Pfender. 1997. Antibiosis and antagonism of Sclerotinia homeocarpa and Drechslera poae by Pseudomonas fluorescens Pf-5 in vitro and in planta. Phytopathology. 87(6):p. 614-621. |
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| Web URL(s):
http://apsjournals.apsnet.org/doi/pdfplus/10.1094/PHYTO.1997.87.6.614
Last checked: 08/26/2010
Requires: PDF Reader |
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MSU catalog number: SB 599 .P48 |
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