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| Web URL(s): | http://apsjournals.apsnet.org/doi/pdf/10.1094/PDIS.1998.82.7.738
Last checked: 08/27/2010
Requires: PDF Reader |
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| Publication Type:
| Refereed |
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| Author(s): | Doss, Robert P.;
Clement, Stephen L.;
Kuy, Srey-Reath;
Welty, Ronald E. |
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| Author Affiliation: | USDA-ARS, Horticultural Crops Research Unit, Corvallis, OR 97330 and Department of Horticulture, Oregon State University, Corvallis 97331; USDA-ARS, Western Regional Plant Introduction Station, Pullman, WA 99164; USDA-ARS, Horticultural Crops Research Unit, Corvallis, OR 97330; and USDA-ARS, National Forage Seed Production Research Unit (retired), Corvallis, OR 97333 |
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| Title: | A PCR-based technique for detection of Neotyphodium endophytes in diverse accessions of tall fescue |
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| Section: | Research
Other records with the "Research" Section
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| Source: | Plant Disease. Vol. 82, No. 7, July 1998, p. 738-740. |
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| Publishing Information: | St. Paul, MN: American Phytopathological Society |
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| # of Pages: | 3 |
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| Keywords: | TIC Keywords: Endophytes; Festuca arundinacea; Sampling techniques; DNA; Polymerase chain reaction; Identification; Genetic markers
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| Abstract/Contents: | "A previously described polymerase chain reaction (PCR)-based method used for detection of Neotyphodium coenophialum in tall fescue detected Neotyphodium endophytes in some, but not all, infected plants from a geographically diverse sample. In the study reported here, a different set of primers, based on intervening sequences of the tubulin 2 gene, were prepared and used for PCR. PCR with these primers yielded the expected 444 base pair amplification product with DNA from 104 of the 106 infected accesssions tested. In addition, one accession originally scored as endophyte-free on the basis of a tissue culture test was correctly rated as endophyte-infected using the PCR procedure. Results suggest that primers based on intervening sequences of the tubulin 2 gene can be used for PCR-based detection of Neotyphodium endophytes in tall fescue accession of diverse origin." |
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| Language: | English |
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| References: | 26 |
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| Note: | Figures
Tables |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Doss, R. P., S. L. Clement, S.-R. Kuy, and R. E. Welty. 1998. A PCR-based technique for detection of Neotyphodium endophytes in diverse accessions of tall fescue. Plant Dis. 82(7):p. 738-740. |
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| Web URL(s):
http://apsjournals.apsnet.org/doi/pdf/10.1094/PDIS.1998.82.7.738
Last checked: 08/27/2010
Requires: PDF Reader |
| 
MSU catalog number: SB 599 .P95 |
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