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| Web URL(s): | https://archive.lib.msu.edu/tic/its/articles/1997jou283.pdf
Last checked: 09/29/2008
Requires: PDF Reader |
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| Access Restriction: | Certain MSU-hosted archive URLs may be restricted to legacy database members. |
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| Publication Type:
| Refereed |
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| Author(s): | Fei, S.;
Read, P. E.;
Riordan, T. P. |
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| Author Affiliation: | Department of Horticulture, University of Nebraska-Lincoln, Nerbraska USA |
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| Title: | In vitro regeneration of Buchloe dactyloides (Nutt.) Engelm. through immature inflorescence culture |
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| Section: | Breeding and genetics: Contributed papers
Other records with the "Breeding and genetics: Contributed papers" Section
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| Meeting Info.: | Sydney, Australia: 1997 |
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| Source: | International Turfgrass Society Research Journal. Vol. 8, No. Part 1, 1997, p. 283-289. |
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| Publishing Information: | Blacksburg, VA: International Turfgrass Society |
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| # of Pages: | 7 |
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| Keywords: | TIC Keywords: Bouteloua dactyloides; Regeneration; In vitro; Calli; Genetic variability; Comparisons; Inflorescences
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| Abstract/Contents: | "Immature inflorescences taken from the vegetatively propagated female cultivar, '609', were cultured on Murashige and Skoog (MS) medium containing 1, 2, 3 or 4 mg 2,4-D lā»Ā¹ combined with BA at 0, 0.2 or 0.4 mg 1ā»Ā¹. Yellow-white callus with nodular structures was formed in all treatments after a 4-week culture in the dark at 25Ā°C. Only calli that formed on medium supplemented with 2,4-D alone at 1, 2 or 3 mg 1ā»Ā¹ were regenerable under our experimental conditions. 2,4-D at 4 mg 1ā»Ā¹ suppressed callus formation. Callus that formed under constant light became mucilaginous after 10 days without formation of any nodular structures, while those produced under dark culture did not become mucilaginous until 3 weeks after culture when the nodular structures were visible. About 17% of the calli produced shoots when they were transferred to MS medium supplemented with 0.1 mg BA 1ā»Ā¹. Shoots were then transferred to MS medium containing NAA 0.3 mg 1āā where roots formed after 1 week. Inflorescences collected in early spring had a higher percentage of callus formation than those collected in the autumn. Two other cultivars, a female, '315', and a male, NE84-45-3, were tested to compare genotype effect. The male cultivar, NE84-45-3 exhibited higher percentage callus formation and regenerability than was found for the other two cultivars." |
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| Language: | English |
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| References: | 15 |
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| Note: | Pictures, b/w
Figures
Tables |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Fei, S., P. E. Read, and T. P. Riordan. 1997. In vitro regeneration of Buchloe dactyloides (Nutt.) Engelm. through immature inflorescence culture. Int. Turfgrass Soc. Res. J. 8(Part 1):p. 283-289. |
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| Web URL(s):
https://archive.lib.msu.edu/tic/its/articles/1997jou283.pdf
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MSU catalog number: SB 433 .I52 v. 8 |
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