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| Author(s): | Huff, David R.;
Bara, Janice M. |
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| Author Affiliation: | Department of Plant Science, Cook College, Rutgers University, New Brunswick, NJ 08903-0231 |
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| Title: | Determining genetic origins of aberrant progeny from facultative apomictic Kentucky bluegrass using a combination of flow cytometry and silver-stained RAPD markers |
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| Source: | Theoretical and Applied Genetics. Vol. 87, No. 1/2, October 1993, p. 201-208. |
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| Publishing Information: | Berlin: Springer |
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| # of Pages: | 8 |
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| Keywords: | TIC Keywords: Genes; Relationships; Ploidy; Identification; Methodology; Random amplified polymorphic DNA markers; Poa pratensis
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| Abstract/Contents: | "Seeded plants that reproduce through facultative apomixis produce two types of progeny: (1) apomictic progeny genetically identical to the maternal genotype, and (2) aberrant progeny genetically different from the maternal genotype. Aberrant progeny complicate the results of basic and applied genetic studies. Determining the genetic origin of progeny plants using traditional techniques, such as cytology, embryology, and segregational studies, is technically difficult in Kentucky bluegrass. We have found that two relatively new techniques, flow cytometry and silver-stained RAPD (ssRAPD) markers, are powerful tools for rapidly determining the genetic origins of aberrant Kentucky bluegrass progeny. Our application of these techniques demonstrate that (1) flow cytometry accurately distinguishes progeny ploidy levels, and (2) ssRAPD markers distinguish progeny resulting from cross-fertilization. Therefore, a combination of flow cytometry and ssRAPD data would be useful for most genetic studies of aberrant individuals. Moreover, ssRAPDs were found to be of value for measuring the loss of genetic markers from polyhaploids and quantifying the inheritance of parental genomes in polyploid B^D[I^D[I (n + n) and polytriploid B^D[I^D[I^D[I (2n + n) hybrids. Quantifying shared ssRAPD markers may also be useful for determining genetic relatedness between varieties and germplasm sources." |
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| Language: | English |
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| References: | 30 |
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| Note: | Pictures, b/w
Tables
Graphs |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Huff, D. R., and J. M. Bara. 1993. Determining genetic origins of aberrant progeny from facultative apomictic Kentucky bluegrass using a combination of flow cytometry and silver-stained RAPD markers. Theor. Appl. Genet. 87(1/2):p. 201-208. |
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