| |
| Publication Type:
| Report |
|---|
| Content Type: | Abstract or Summary only |
|---|
| Author(s): | Obermeier, M. R.;
Avidiushko, S. A.;
Barret, M. |
|---|
| Author Affiliation: | Department of Agronomy, University of Kentucky, Lexington, KY 40546-0091 |
|---|
| Title: | Comparative sequence of the acetyl CoA binding site of acetyl CoA carboxylase genes isolated from accase inhibitor resistant and susceptible johnsongrass biotypes |
|---|
| Meeting Info.: | 51st Annual Meeting, Birmingham, AL, January 26-28, 1998 |
|---|
| Source: | Southern Weed Science Society Proceedings. Vol. 51, January 1998, p. 248-49. |
|---|
| Publishing Information: | Champaign, IL: Southern Weed Science Society. |
|---|
| # of Pages: | 2 |
|---|
| Keywords: | TIC Keywords: Sorghum halepense; Herbicide resistance; Genetic factors; Acetyl-coa carboxylase; Fluazifop; Sethoxydim; Zea mays; Genetic markers; Comparisons
|
|---|
| Abstract/Contents: | "Johnsongrass biotypes resistant to acetyl CoA carboxylase (ACCase) inhibitor herbicides have been identified in Kentucky and Mississippi. Greenhouse studies with three of the resistant biotypes indicated a higher level of resistance to the aryloxyphenoxypropionate (AOPP) herbicide fluazifop-P compared to the cyclohexanedione (CHD) herbicide sethoxydim. More detailed laboratory research with a resistant biotype from Kentucky indicated resistance is likely due to decreased ACCase sensitivity to fluazifop-P. Degenerate primers flanking the acetyl CoA binding site of ACCase were designed based on homology between a number of published plant ACCase sequences and used with RT-PCR protocols to obtain sequences. Acetyl CoA binding region sequences from the resistant johnsongrass biotypes were compared to each other and to the sequences of four susceptible johnsongrass populations. For further comparison, the acetyl CoA binding site from a sethoxydin resistant corn variety was sequenced. The isolated acetyl CoA binding region sequences showed either homology to published plastidic ACcase sequences (3 resistant and 4 susceptible johnsongrass biotypes and sethoxydim resistant corn) or to cytosolic ACCase sequences (2 resistant johnsongrass). Although variations in the sequences do occur, no consistent pattern between resistant and susceptible johnsongrass biotypes was seen. Most likely the mutation(s) related to resistance is not located in the acetyl CoA binding region ACCase. Additionally, previous research has indicated the mutation(s) related to resistance is not located in the carboxybiotin binding region of ACCase." |
|---|
| Language: | English |
|---|
| References: | 0 |
|---|
| Note: | This item is an abstract only! |
|---|
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Obermeier, M. R., S. A. Avidiushko, and M. Barret. 1998. Comparative sequence of the acetyl CoA binding site of acetyl CoA carboxylase genes isolated from accase inhibitor resistant and susceptible johnsongrass biotypes. South. Weed Sci. Soc. Proc. 51:p. 248-49. |
| Fastlink to access this record outside TGIF: https://tic.msu.edu/tgif/flink?recno=57456 |
| If there are problems with this record, send us feedback about record 57456. |
| Choices for finding the above item: |
|---|
| 
MSU catalog number: SB 611 .S6 v.51 |
| Find from within TIC:
Digitally in TIC by file name: swssp2003 |
| Request through your local library's inter-library loan service (bring or send a copy of this TGIF record) |
