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| Web URL(s): | http://onlinelibrary.wiley.com/doi/10.1111/j.1744-7348.1981.tb05114.x/epdf
Last checked: 09/29/2015
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| Publication Type:
| Refereed |
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| Author(s): | Torrance, Lesley;
Harrison, B. D. |
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| Author Affiliation: | Torrance: Ministry of Agriculture, Fisheries and Food, Harpenden Laboratory, Hatching Green, Harpenden, Herts.; Harrison: Scottish Crop Research Institute, Invergowrie, Dundee |
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| Title: | Properties of Scottish isolates of cocksfoot mild mosaic virus and their comparison with others |
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| Source: | Annals of Applied Biology. Vol. 97, No. 3, April 1981, p. 285-295. |
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| # of Pages: | 11 |
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| Keywords: | TIC Keywords: Phleum pratense; Cocksfoot mild mosaic virus
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| Geographic Terms: | UK |
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| Abstract/Contents: | Two isolates from Dactylis glomerata in Scotland differed in symptoms and host range from those obtained in Wales and the German Federal Republic. They were serologically more closely related to a Dutch isolate from D. glomerata and to a Scottish isolate from Phleum pratense than was the German isolate from D. glomerata. The Scottish isolate from P. pratense was more virulent than, but serologically closely related to, a Welsh isolate from P. pratense. Particles of the Scottish isolates from both hosts were best preserved from electron microscopy by fixation with osmium tetroxide. In 1 M KCl or 0.01 M ethylene diamine tetraacetate they were stable at pH 5.2-5.3 but unstable above pH 7; they were disrupted by 0.5% sodium dodecyl sulphate, The particles contained major and minor RNA components of mol. wt. c. 1.5 X 106 (RNA-1) and 0.5 X 106 (RNA-21), respectively, with polydisperse RNA of intermediate mol. wt. and protein of mol. wt. c. 27 000. In CsCl gradients, major and minor nucleoprotein components of density 1.39 and 1.38 g/ml respectively were distinguished. The less dense particles contained a larger proportion of intermediate sized RNA molecules and of RNA-2, and a smaller proportion of RNA-1, than did the denser particles. Particles seemed to contain either RNA-1 or various combinations of smaller RNA molecules. Despite the differences in antigenic constitution, symptoms and particle stability between isolates obtained from the 2 spp. in different countries, these isolates seem sufficiently similar to be considered 1 virus. |
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| Language: | English |
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| References: | 20 |
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| Note: | Pictures, b/w
Tables
Graphs |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Torrance, Lesley, and B. D. Harrison. 1981. Properties of Scottish isolates of cocksfoot mild mosaic virus and their comparison with others. Ann. Appl. Biol. 97(3):p. 285-295. |
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| Web URL(s):
http://onlinelibrary.wiley.com/doi/10.1111/j.1744-7348.1981.tb05114.x/epdf
Last checked: 09/29/2015
Requires: PDF Reader
Access conditions: Item is within a limited-access website |
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MSU catalog number: QH 301 .A48 |
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