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| Publication Type:
| Report |
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| Author(s): | Han, D. Y.;
Rimelspach, J. W.;
Boehm, M. J. |
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| Author Affiliation: | Department of Plant Pathology, The Ohio State University |
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| Title: | Evaluation of Pseodomonas aureofaciens strain TX-1 for control of dollar spot on creeping bentgrass, 1999 |
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| Section: | Turfgrass
Other records with the "Turfgrass" Section
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| Source: | Biological and Cultural Tests for Control of Plant Diseases. 2000, p. 48-49. |
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| Publishing Information: | St. Paul, MN: The American Phytopathological Society |
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| # of Pages: | 2 |
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| Keywords: | TIC Keywords: Agrostis stolonifera; Dollar spot; Sclerotinia homoeocarpa; Pseudomonas aureofaciens; Biological control; Disease control; Chlorothalonil; Percent living ground cover; Application rates
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| Abstract/Contents: | "Plots were established at The Ohio State University Turfgrass Research and Education Center In Columbus to evaluate the ability of the bacterial biocontrol agent Pseudomonas aureofaciens strain TX-1 to suppress dollar spot. Plots (4 ft x 6 ft) were sprayed 6 nights per wk at sunset with known concentrations of TX-1. TX-1 was grown on site daily in a BioJect® fermentor for 14 h. At the end of the growth cycle, TX-1 suspensions were removed and diluted in tap water to concentrations of 10⁵, 10⁶, 10⁷ and 10⁸ CFU / ml. TX-1 suspensions (500 ml per plot) were applied with a hand sprayer equipped with TeeJet 8010 nozzles charged with compressed N operating at 40 psi. The populations of TX-1 in each of the suspensions were enumerated each week by standard dilution plating on tryticase soy agar amended with rifampicin (50 μg per L). To investigate the influence of turfgrass fertility status on biocontrol agent efficacy, each TX-1 suspension was applied to plots maintained at 1, 2 and 4 lb N / 1000 ft² per season. Nitrogen was applied on 7 May, 27 May, 17 Jun, and 28 Jul. The 1 lb N plots received 0.5 lb N / 1000 ft² on 27 May and 28 Jul only, while the 2 lb and 4 lb N plots received 0.5 and 1 lb N / 1000 ft², respectively, on each application date. Non-TX-1 and fungicide (chlorothalonil as Daconil Ultrex, 3.0 oz per 1000 ft²) plots served as controls. There were 18 treatments in total. Plots were arranged in a randomized complete block design with four replications per treatment. The percent diseased turf was assessed by laying a grid consisting of 570 squares (2 x 2 in.) over each plot and counting the number of symptomatic squares. Percentage data were arcsin transformed and analyzed by ANOVA. Where significant differences were observed, means were separated with Fisher's Least Significant Difference. Dollar spot developed in Jun and Jul even though the weather was unusually hot and dry. At the lower two fertilizer rates, little if any reduction of dollar spot severity was seen with any concentration of TX-1, and no significant differences were observed. At the 4 lb N rate, reductions in dollar spot severity were observed, although this was only significant on June 24. No significant differences were observed between the different concentrations of TX-1 at this fertilizer rate. Evaluation of season-long effects by calculation of areas under disease progress curves for the 4 lb rate revealed significant differences in total dollar spot between nontreated control and TX-1 treated plots." |
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| Language: | English |
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| References: | 0 |
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| Note: | Tables |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Han, D. Y., J. W. Rimelspach, and M. J. Boehm. 2000. Evaluation of Pseodomonas aureofaciens strain TX-1 for control of dollar spot on creeping bentgrass, 1999. Biol. Cult. Tests Control Plant Dis. p. 48-49. |
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