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| Web URL(s): | http://www.turf.rutgers.edu/research/abstracts/symposium1995.pdf#page=15
Last checked: 11/28/2007
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Notes: Item is within a single large file |
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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Frederick, Barbara;
Henson, Joan M. |
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| Author Affiliation: | Department of Microbiology, Montana State University, Bozeman, MT |
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| Title: | Gaeumannomyces Graminis var. Graminis Melanin |
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| Section: | Oral presentations
Other records with the "Oral presentations" Section
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| Meeting Info.: | Cook College, Rutgers, NJ: January 5-6, 1995 |
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| Source: | Proceedings of the Fourth Annual Rutgers Turfgrass Symposium. 1995, p. 17. |
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| Publishing Information: | New Brunswick, NJ: Center for Turfgrass Science, Cook College, Rutgers, The State University of New Jersey |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Gaeumannomyces graminis var. graminis; Copper sulfate; Tricyclazole; Cytology
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| Abstract/Contents: | "Gaeumannomyces graminis var. graminis, a filamentous, soil Ascomycete, synthesized more melanin when exposed to as little as 0.01 mM CuSO₄ in minimal broth culture. Because its synthesis was inhibited by tricyclazole, melanin produced in response to copper was dihydroxynaphthalene (DHN) melanin. An additional hyphal cell wall layer was visualized by electron microscopy of hyphae treated with copper and fixed by cryotechniques. This electron dense layer was between the outer cell wall and the inner chitin layer and doubled the total wall thickness. In copper-grown cells that were also treated with tricyclazole this layer was absent. Hyphopodia grown in copper medium had a melanin layer twice as thick as hyphopodia grown without copper. In addition, hyphopodia grown in copper, unlike those grown without copper, were capable of penetrating mylar memberanes. A silver enhancement method to determine the cellular location of CuS was developed. Silver staining demonstrated that in Cu-treated, melanized cultures, CuS was present in cell walls and septa of hyphae, and in hyphopodia. Electron microscopy of silver stained cells suggested that CuS was associated with the melanin layer of cell walls. G. graminis mutants that produced either more or less melanin were isolated and results of pathogenicity tests on rice will be presented." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Frederick, B., and J. M. Henson. 1995. Gaeumannomyces Graminis var. Graminis Melanin. Proc. Annu. Rutgers Turfgrass Symp. p. 17. |
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| Web URL(s):
http://www.turf.rutgers.edu/research/abstracts/symposium1995.pdf#page=15
Last checked: 11/28/2007
Requires: PDF Reader
Notes: Item is within a single large file |
| 
MSU catalog number: SB 433 .R88 |
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