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| Web URL(s): | http://www.turf.rutgers.edu/research/abstracts/symposium1995.pdf#page=17
Last checked: 11/28/2007
Requires: PDF Reader
Notes: Item is within a single large file |
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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Kobayashi, D.;
El-Barrad, N. |
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| Author Affiliation: | Department of Plant Pathology, Rutgers University, New Brunswick, NJ |
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| Title: | Biological control of summer patch disease |
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| Section: | Oral presentations
Other records with the "Oral presentations" Section
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| Meeting Info.: | Cook College, Rutgers, NJ: January 5-6, 1995 |
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| Source: | Proceedings of the Fourth Annual Rutgers Turfgrass Symposium. 1995, p. 20. |
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| Publishing Information: | New Brunswick, NJ: Center for Turfgrass Science, Cook College, Rutgers, The State University of New Jersey |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Biological control; Disease control; Summer patch; Poa pratensis; Magnaporthe poae; Bacteria; Disease severity; Serratia marcescens; Stenotrophomonas maltophilia; Chitinase
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| Abstract/Contents: | "An enrichment culture procedure was developed to isolate potential biological control agents for summer patch disease of Kentucky bluegrass, caused by Magnaporthe poae. The enrichment procedure involved continuous growth of bacteria in a minimal salts medium supplemented with mycelia of M. poae as a sole carbon source. Using this method, several bacteria were recovered that were capable of suppressing summer patch disease in growth chamber studies. All bacteria that significantly reduced disease were found to express activity of one or more of the following degradative enzymes: chitinase, glucanase, protease or lipase. In addition, all isolates were capable of colonizing roots of Kentucky bluegrass significantly at high levels of greater than 10⁴ colony forming units (CFU)/g root tissue. Two chitinolytic bacteria, Serratia marcescens 9M5 and Xanthomonas maltophilia 21C6, reduced summer patch disease by more than 50% and 70%, respectively. Disease suppression by these isolates was significantly affected by bacterial inoculum concentrations between 10⁸ and 10¹⁰ CFU/ml. Increased concentrations of X. maltophilia resulted in higher levels of disease control. Similarly, disease control increased as concentrations of S. marcescens increased from 10⁸ CFU/ml to 10⁹ CFU/ml. However, at concentrations above 10⁹ CFU/ml, an inverse relationship was observed between levels of disease suppression and concentrations of S. marcescens. Populations of these bacteria in the soil over a 35 day period were directly influenced by inoculum concentration. In contrast, populations within the turfgrass rhizosphere appeared not to be influenced by inoculum concentration. The role of chitinase in disease suppression by both X. maltophilia 21C6 and S. marcescens 9M5 was assessed. The chiA gene cloned from S. marcescens 9M5 was mobilized into BF92-14, a previously identified, root-colonizing bacterium with summer patch suppressive ability. Expression of chiA in BF92-14 improved levels of disease control by this bacterium. In related studies, mutagenesis of the chitinase gene from X. maltophilia 21C6 resulted in less disease control compared to wildtype strain. The role of chitinase, combined with the effect of bacterial concentration on disease control and bacterial populations, provide supporting evidence that disease suppression is a result of a direct effect of these bacteria on pathogen inoculum within the soil." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Kobayashi, D., and N. El-Barrad. 1995. Biological control of summer patch disease. Proc. Annu. Rutgers Turfgrass Symp. p. 20. |
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| Web URL(s):
http://www.turf.rutgers.edu/research/abstracts/symposium1995.pdf#page=17
Last checked: 11/28/2007
Requires: PDF Reader
Notes: Item is within a single large file |
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MSU catalog number: SB 433 .R88 |
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