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| Web URL(s): | https://acsess.onlinelibrary.wiley.com/doi/10.2136/sssaj2001.1734
Last checked: 02/28/2024
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https://acsess.onlinelibrary.wiley.com/doi/epdf/10.2136/sssaj2001.1734
Last checked: 02/28/2024
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Access conditions: Item is within a limited-access website |
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| Publication Type:
| Refereed |
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| Author(s): | Wright, S. F.;
Jawson, L. |
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| Author Affiliation: | USDA-ARS - Sustainable Agricultural Systems Laboratory, Beltsville, MD |
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| Title: | A pressure cooker method to extract glomalin from soils |
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| Section: | Division S-3---Soil biology & biochemistry
Other records with the "Division S-3---Soil biology & biochemistry" Section
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| Source: | Soil Science Society of America Journal. Vol. 65, No. 6, November/December 2001, p. 1734-1735. |
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| Publishing Information: | Madison, WI: Soil Science Society of America |
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| # of Pages: | 2 |
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| Keywords: | TIC Keywords: Methodology; Proteins; Mycorrhizal fungi; Temperatures; Pressure
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| Abstract/Contents: | "Glomalin, a glycoprotein produced by arbuscular mycorrhizal fungi, is an important constituent of soil organic matter. Glomalin is insoluble in water or salt solutions commonly used in soil extractions. Solubiliztion of glomalin does occur in the presence of citrate at the temperature achieved by an autoclave (121°C, 103 kPa). Most soil-testing laboratories have access to all of the instruments required for glomalin analysis except an autoclave. Small (4-L) and large (14-L) pressure cookers were compared with a bench-top autoclave to test glomalin extraction from three soils using 50 mM citrate, pH 8.0 as the extracting agent. Glomalin concentrations, as measured by the Bradford protein assay, were identical for extracts from the autoclave and the 14-L pressure cooker when 103 kPa (121°C) was achieved in both vessels. The 4-L pressure cooker extracted less glomalin than the other vessels. A pressure cooker that will achieve 103 kPa is a low-cost substitute for an autoclave to extract glomalin from soils." |
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| Language: | English |
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| References: | 6 |
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| Note: | Graphs |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Wright, S. F., and L. Jawson. 2001. A pressure cooker method to extract glomalin from soils. Soil Sci. Soc. Am. J. 65(6):p. 1734-1735. |
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| Web URL(s):
https://acsess.onlinelibrary.wiley.com/doi/10.2136/sssaj2001.1734
Last checked: 02/28/2024
Access conditions: Item is within a limited-access website
https://acsess.onlinelibrary.wiley.com/doi/epdf/10.2136/sssaj2001.1734
Last checked: 02/28/2024
Requires: PDF Reader
Access conditions: Item is within a limited-access website |
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MSU catalog number: S 590 .S65 |
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