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| DOI: | 10.1002/ps.543 |
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| Web URL(s): | http://onlinelibrary.wiley.com/doi/10.1002/ps.543/full
Last checked: 01/31/2014
Access conditions: Item is within a limited-access website
http://onlinelibrary.wiley.com/doi/10.1002/ps.543/pdf
Last checked: 01/31/2014
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| Publication Type:
| Refereed |
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| Author(s): | Ziogas, Basil N.;
Markoglou, Anastasios N.;
Tzima, Aliki |
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| Author Affiliation: | Laboratory of Plant Pathology, Agricultural University of Athens, Votanikos, Athens, Greece |
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| Title: | A non-Mendelian inheritance of resistance to strobilurin fungicides in Ustilago maydis |
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| Source: | Pest Management Science. Vol. 58, No. 9, September 2002, p. 908-916. |
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| Publishing Information: | London: for the Society of Chemical Industry by John Wiley & Sons |
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| # of Pages: | 9 |
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| Related Web URL: | http://onlinelibrary.wiley.com/doi/10.1002/ps.543/abstract
Last checked: 01/31/2014
Notes: Abstract only |
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| Keywords: | TIC Keywords: Azoxystrobin; Cytoplasmic inheritance; Fungicide resistance; Mutants; Ustilago
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| Abstract/Contents: | "Mutants of Ustilago maydis (DC) Corda with high resistance to azoxystrobin (RF 164 to 4714, based on EC50 values), an inhibitor of mitochondrial electron transport at the cytochrome bc1 complex, were isolated in a mutation frequency of 2.3 x 10 -7 after nitrosoguanidine mutagenesis and selection on media containing 1 μugml-1 azoxystrobin in addition to 0.5 mM salicylhydroxamate (SHAM), a specific inhibitor of cyanide-resistant (alternative) respiration. Oxygen uptake in whole cells was strongly inhibited in the wild-type strains by azoxystrobin (1.5 μugml-1) in addition to SHAM (1mM), but not in the mutant isolates. Genetic analysis with nine such mutant isolates resulted in progeny phenotypes which did not follow Mendelian segregation, but satisfied the criteria of non-Mendelian (cytoplasmic) heredity. In crosses between three mutant isolates with the compatible wild-type strains, the sensitivity was inherited by progeny maternally from the wild-type parent strain (criterion of uniparental inheritance). In crosses between wild-type strains and reamining mutant isolates, a continuous distribution of sensitivity in the progeny was found (criterion of vegetative segregation). The third criterion of cytoplasmic resistantce (criterion of intracellular selection) was fulfilled by experiments on the stability of resistance phenotypes. With two exceptions, a reduction of resistance was observed in the mutant strains when they were grown on inhibitor-free medium. Recovery of the high resistance level was observed after they were returned to the selection medium. Cross-resistance studies with other fungicides, which also inhibit electron transport through complex III of respiratory chain, showed that mutations for resistance to azoxystrobin were also responsible for reduced sensitivity to kresoxim-methyl (RF 18 to 1199) and to antimycin-A (RF 20 to 305), which act at the Qo and Qi sites of the cytochrome bc1 complex, respectively. Studies of the fitness of azoxystrobin-resistant isolates showed that these mutations appeared to be pleitropic, having significant adverse effects on growth in liquid culture and pathogenicity of young corn plants." |
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| Language: | English |
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| References: | 44 |
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| Note: | Tables
Graphs |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Ziogas, B. N., A. N. Markoglou, and A. Tzima. 2002. A non-Mendelian inheritance of resistance to strobilurin fungicides in Ustilago maydis. Pest Manage. Sci. 58(9):p. 908-916. |
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| DOI: 10.1002/ps.543 |
| Web URL(s):
http://onlinelibrary.wiley.com/doi/10.1002/ps.543/full
Last checked: 01/31/2014
Access conditions: Item is within a limited-access website
http://onlinelibrary.wiley.com/doi/10.1002/ps.543/pdf
Last checked: 01/31/2014
Requires: PDF Reader
Access conditions: Item is within a limited-access website |
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MSU catalog number: SB 951 .A1 P45 |
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