Full TGIF Record # 95093
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DOI:10.1002/ps.827
Web URL(s):http://onlinelibrary.wiley.com/doi/10.1002/ps.827/full
    Last checked: 02/07/2014
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http://onlinelibrary.wiley.com/doi/10.1002/ps.827/pdf
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Publication Type:
i
Refereed
Author(s):Abosriwil, Salem O.; Clancy, Kevin J.
Author Affiliation:Department of Environmental Resource Management, Faculty of Agriculture, University College Dublin, Belfield, Dublin, Ireland
Title:A mini-bag technique for evaluation of fungicide effects on Trichoderma spp in mushroom compost
Source:Pest Management Science. Vol. 60, No. 4, April 2004, p. 350-358.
Publishing Information:London: for the Society of Chemical Industry by John Wiley & Sons
# of Pages:9
Related Web URL:http://onlinelibrary.wiley.com/doi/10.1002/ps.827/abstract
    Last checked: 02/07/2014
    Notes: Abstract only
Keywords:TIC Keywords: Trichoderma; Composts; Mushroom compost; Trichoderma viride; Inoculum; Fungicides; Laboratory methods
Abstract/Contents:"An in vivo technique was developed to observe colonisation of mushroom compost by Trichoderma spp. Isolates of T harzianum (Th2), T harzianum (Th1), T koningii (Tk) and T viride (Tv) were artificially introduced into compost using a mini-bag system. Wheat grains, colonised by Trichoderma spp, were placed centrally on a layer of compost at the bottom of 1-litre polythene bags which were then filled with 350 g of spawned or un-spawned compost, and partially sealed. After 14 and 21 days incubation at 27°C, the bags were assessed for recovery of Trichoderma from middle and top zones using a needle stab re-isolation technique and a visual colonisation scoring system. Visible green mould contamination, similar to that observed in practice, developed within 21 days. The visual colonisation scoring was reliably related to the re-isolation success. In this evaluation, Trichoderma spp showed considerable differences in their relative abilities to colonise spawned and un-spawned compost, with Th2 isolates being consistently superior to the other isolates of Th1, Tk and Tv in colonising spawned compost. This technique was employed to evaluate the effects of fungicides on the colonisation of mushroom compost by three Trichoderma spp: Th2, Th1 and Tk, using 1-litre and 5-litre mini-bag systems. Aqueous suspensions of benomyl, carbendazim, thiabendazole, prochloraz and prochloraz + carbendazim incorporated into the compost at 50 mg litre-1, or applied to spawn at 50 mg kg-1, reduced the colonisation by Trichoderma spp. Prochloraz and prochloraz + carbendazim were superior to benomyl, carbendazim or thiabendazole in reducing compost colonisation by Th2, Th1 and Tk, with Th2 being the most persistent type, capable of colonising treated compost in the presence of all five fungicides. The prochloraz + carbendazim mixture, not normally used in mushroom production, was equal to or better than prochloraz alone. The incidence of green mould colonisation by Th2 was as extensive in the 5-litre compost bags as in the 1-litre bags, but colonisation by Th1 and Tk was more apparent in the 5-litre bags. The in vivo mini-bag evaluations using wheat grain Trichoderma inoculum and needle stab re-isolation procedures proved an efficient method for studying colonisation and screening for effectiveness of fungicides applied to mushroom compost or spawn."
Language:English
References:26
Note:Pictures, b/w
Tables
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Abosriwil, S. O., and K. J. Clancy. 2004. A mini-bag technique for evaluation of fungicide effects on Trichoderma spp in mushroom compost. Pest Manage. Sci. 60(4):p. 350-358.
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DOI: 10.1002/ps.827
Web URL(s):
http://onlinelibrary.wiley.com/doi/10.1002/ps.827/full
    Last checked: 02/07/2014
    Access conditions: Item is within a limited-access website
http://onlinelibrary.wiley.com/doi/10.1002/ps.827/pdf
    Last checked: 02/07/2014
    Requires: PDF Reader
    Access conditions: Item is within a limited-access website
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MSU catalog number: SB 951 .A1 P45
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