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Web URL(s): | http://www.apsnet.org/publications/phytopathology/backissues/Documents/1988Articles/Phyto78n02_237.pdf Last checked: 10/15/2015 Requires: PDF Reader |
Publication Type:
| Refereed |
Author(s): | Saha, D. C.;
Jackson, M. A.;
Johnson-Cicalese, J. M. |
Author Affiliation: | Dept. of Soils and Crops, New Jersey Agricultural Experiment Station, New Brunswick, NJ. |
Title: | A Rapid Staining Method for Detection of Endophytic Fungi in Turf and Forage Grasses |
Source: | Phytopathology. Vol. 78, No. 2, February 1988, p. 237-239. |
Publishing Information: | St. Paul, MN: American Phytopathological Society |
Keywords: | TIC Keywords: Techniques; Endophytic fungi; Disease identification
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Abstract/Contents: | "Because of the increased interest in endophyte-infected grasses, an efficient detection method was deemed necessary. To facilitate screening a large number of individual seeds and plants, a rapid, safe staining procedure was developed using rose bengal. Previous methods were time-consuming and often required careful boiling of the plant tissues. The rose bengal staining prcedure is simple and effective and gave excellent visual results. Minimal staining times of 30-60 sec gave good fungal differentiation even in thick tissues, and no additional fixing was necessary. Seeds and dried tissue also were stained effectively." |
Language: | English |
References: | 12 |
Note: | Pictures: Color Tables |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Saha, D. C., M. A. Jackson, and J. M. Johnson-Cicalese. 1988. A Rapid Staining Method for Detection of Endophytic Fungi in Turf and Forage Grasses. Phytopathology. 78(2):p. 237-239. |
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| Web URL(s): http://www.apsnet.org/publications/phytopathology/backissues/Documents/1988Articles/Phyto78n02_237.pdf Last checked: 10/15/2015 Requires: PDF Reader |
| MSU catalog number: SB 599 .P48 |
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