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Web URL(s): | http://www.actahort.org/books/783/783_28.htm Last checked: 06/10/2008 Access conditions: Item is within a limited access website |
Publication Type:
| Report |
Author(s): | Liebao, H.;
Li, Xue;
Liu, Jun;
Zeng, Huiming |
Title: | Droughttolerant transgenic perennial ryegrass (Lolium perenne L.) obtained via particle bombardment gene transformation of CBF3/DREB1A gene |
Meeting Info.: | Proceedings of the II International Conference on Turfgrass Science and Management for Sports Fields: Beijing, China, June 24-29, 2007. |
Source: | Acta Horticulturae. Vol. 783, February 2008, p. 273-282. |
Publishing Information: | Leuven, Belgium: International Society for Horticultural Science |
# of Pages: | 10 |
Keywords: | TIC Keywords: Drought resistance; Lolium perenne; Particle bombardment; Genetic transformation; Transgenic turfgrasses
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Abstract/Contents: | "Embryogenic calluses, initiated from mature embryos of three cultivars were transformed with pCAMBIA1301 containing ß-glucuronidase (GUS) gene, hygromycin phosphstransferase (HPT) gene, and the Arabidopsis CBF3/DREB1A gene by bombardment. To screen the putative transgenic plants, subsequent selection in the presence of a high concentration of hygromycin was applied at the tissue culture stage (100 mg L-1) and in the regeneration medium (50 mg L-1). In hygromycin-tolerant plants, genomic integration of CBF3 was confirmed by PCR amplification followed by Southern and Northern analyses. All the transgenic plants grew, and developed into sexual stage naturally under the greenhouse and field conditions. Transgenic and control plants of two cultivars were exposed to drought stress in greenhouse until they became brown and completely desiccated (16 d). Transgenic plants from two cultivars both exhibited lower electrolyte leakage (EL), malondialdehyde (MDA), and peroxidase (POD) activity than did control plants along with the decline of the soil moisture content (SMC). The chlorophyll content and superoxide dismutase (SOD) activities in transgenic plants were higher than that in control plants when the SMC declined. The adverse impact of the drought stress was more severe for control plants than for transgenic plants. CBF3/DREB1A transgenic ryegrass with enhanced drought tolerance suggested that the overexpression of the CBF3 gene serves as a novel strategy to produce drought-tolerant grasses." |
Language: | English |
References: | 0 |
See Also: | Other items relating to: Breeding for Drought |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Liebao, H., X. Li, J. Liu, and H. Zeng. 2008. Droughttolerant transgenic perennial ryegrass (Lolium perenne L.) obtained via particle bombardment gene transformation of CBF3/DREB1A gene. Acta Horticulturae. 783:p. 273-282. |
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| Web URL(s): http://www.actahort.org/books/783/783_28.htm Last checked: 06/10/2008 Access conditions: Item is within a limited access website |
| MSU catalog number: SB 13 .A25 no. 783 |
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