| |
DOI: | 10.21273/HORTSCI.44.7.2049 |
Web URL(s): | https://journals.ashs.org/hortsci/view/journals/hortsci/44/7/article-p2049.xml Last checked: 12/06/2019 Requires: PDF Reader |
Publication Type:
| Refereed |
Author(s): | Wang, Ying;
Bigelow, Cale A.;
Jiang, Yiwei |
Author Affiliation: | Department of Agronomy, Purdue University, West Lafayette, IN |
Title: | Ploidy level and DNA content of perennial ryegrass germplasm as determined by flow cytometry |
Section: | Short communications Other records with the "Short communications" Section
|
Source: | HortScience. Vol. 44, No. 7, December 2009, p. 2049-2052. |
Publishing Information: | Alexandria, VA: American Society for Horticultural Science |
# of Pages: | 4 |
Keywords: | TIC Keywords: DNA; Diploids; Germplasm; Laser flow cytometry; Lolium perenne; Ploidy; Tetraploidy
|
Abstract/Contents: | "Perennial ryegrass (Lolium perenne L.) is a widely used cool-season turfgrass species. The exact ploidy levels of the worldwide perennial ryegrass accessions in the USDA National Plant Germplasm System (NPGS) are unknown, which could complicate future use and breeding efforts. The objective of this study was to determine the ploidy level and DNA content of the 194 USDA NPGS perennial ryegrass accessions and six commercial cultivars (Brightstar SLT, Catalina II, Divine, Inspire, Manhattan 4, Silver Dollar) using flow cytometry. Among the 200 accessions, 194 diploids and six tetraploids were identified. Three tetraploids originated from Canada with the remaining from Ireland, Japan, and The Netherlands. The average DNA content was 5.60 pg/2C for the diploid and 11.45 pg/2C for the tetraploid. The 2C DNA content was positively correlated (r = 0.23, P < 0.01) with seedling plant height but not seedling leaf width. This ploidy data provide important information for future marker trait analysis and cultivar improvement." |
Language: | English |
References: | 16 |
Note: | Tables Graphs |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Wang, Y., C. A. Bigelow, and Y. Jiang. 2009. Ploidy level and DNA content of perennial ryegrass germplasm as determined by flow cytometry. HortScience. 44(7):p. 2049-2052. |
| Fastlink to access this record outside TGIF: https://tic.msu.edu/tgif/flink?recno=158817 |
| If there are problems with this record, send us feedback about record 158817. |
| Choices for finding the above item: |
| DOI: 10.21273/HORTSCI.44.7.2049 |
| Web URL(s): https://journals.ashs.org/hortsci/view/journals/hortsci/44/7/article-p2049.xml Last checked: 12/06/2019 Requires: PDF Reader |
| MSU catalog number: b2217685a |
| Find from within TIC: Digitally in TIC by record number. |
| Request through your local library's inter-library loan service (bring or send a copy of this TGIF record) |