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Web URL(s): | http://turf.rutgers.edu/research/abstracts/symposium2016.pdf#page=45 Last checked: 07/20/2016 Requires: PDF Reader Notes: Item is within a single large file |
Publication Type:
| Report |
Author(s): | Averello, Vincenzo;
Kubik, Christine;
Vaiciunas, Jennifer;
Bonos, Stacy A.;
Meyer, William A.;
Honig, Josh A. |
Author Affiliation: | Department of Plant Biology and Pathology, Rutgers University |
Title: | Classification of tall fescue (Festuca arundinacea Schreb.) cultivars and collections using chloroplast microsatellite (cpSSR) markers |
Section: | Poster presentations Other records with the "Poster presentations" Section
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Meeting Info.: | New Brunswick, New Jersey: March 18, 2016 |
Source: | Proceedings of the Twenty-Fifth Annual Rutgers Turfgrass Symposium. Vol. 25, 2016, p. 45. |
Publishing Information: | New Brunswick, New Jersey: The Center for Turfgrass Science, Rutgers, The State University of New Jersey |
# of Pages: | 1 |
Keywords: | TIC Keywords: Festuca arundinacea; Gene mapping; Genetic analysis; Genetic diversity; Genetic markers; Microsatellites; Simple sequence repeats
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Abstract/Contents: | "Tall fescue (Festuca arundinacea Schreb.) is a major grass species that has been used extensively as a turfgrass. Turf cultivars of tall fescue are highly suitable for home lawns and sports fields due to their superior drought and heat tolerance and fine texture. Tall fescue occurs in three morphotypes: 1) the continental morphotype that occurs from Northern Europe to the Northern Coast of the Mediterranean Sea; 2) a rhizomatous type that occurs on the Iberian Peninsula; and, 3) the Mediterranean type that occurs predominantly in North Africa. The objective of the current study was to determine the genetic relatedness and evolutionary history of tall fescue and some closely related species using chloroplast microsatellite (cpSSR) markers. Chloroplast SSR markers have been utilized in other grasses including Zea species, Agrostis species, and Oryza sativa L. Chloroplast SSR marker PCR primers were developed from the published chloroplast genome of tall fescue along with one cultivar of perennial ryegrass (Lolium perenne L.), two accessions of annual ryegrass (Lolium multiflorum Lam.), three accessions of tetraploid tall fescue (Festuca arundinacea L. var. glaucescens Boiss), and two accessions of meadow fescue (Festuca pratensis (Huds.) P. Beauv.). Each population was compromised of 16 individuals, and each individual was genotyped using 18 cpSSR markers. Amplicon size was determined by capillary electrophoresis (Applied Biosystems 3500 XL Genetic Analyzer). A total of 85 alleles were identified in the current data set, with 3-8 alleles per locus. A total of 145 haplotypes were identified across all samples, with 109 being private haploytypes (those that only occur in 1 population). The number of haplotypes per population ranged from1 to 9. Analysis of Molecular Variance (AMOVA) indicated that 58% of the total genetic variation was partitioned among populations, while 42% was partitioned within populations. Of 15, 931 interpopulation pairwise comparison, 2873 were not significant (P ā„ 0.05). The cpSSR dendrogram separated the turf and forage tall fescues, as well as each of the species in the current study; however, there was not clear resolution based on breeding history for the turf-type cultivars compared to previous analyses using nuclear microsatellite (nuSSR) markers. Based on this information, cpSSR markers are not capable of distinguishing different cultivars, but could be useful in determining species and tall fescue morphotype." |
Language: | English |
References: | 0 |
Note: | This item is an abstract only! |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Averello, V., C. Kubik, J. Vaiciunas, S. A. Bonos, W. A. Meyer, and J. A. Honig. 2016. Classification of tall fescue (Festuca arundinacea Schreb.) cultivars and collections using chloroplast microsatellite (cpSSR) markers. Proc. Rutgers Turfgrass Symp. 25:p. 45. |
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| Web URL(s): http://turf.rutgers.edu/research/abstracts/symposium2016.pdf#page=45 Last checked: 07/20/2016 Requires: PDF Reader Notes: Item is within a single large file |
| MSU catalog number: b3696858 |
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