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Web URL(s): | https://www.crops.org/publications/cs/pdfs/38/2/CS0380020523 Last checked: 08/05/2010 Requires: PDF Reader Access conditions: Item is within a limited-access website |
Publication Type:
| Refereed |
Author(s): | Bell, G. E.;
McDonald, M. B. Jr.;
Danneberger, T. K.;
St. Martin, S. K. |
Author Affiliation: | Department of Horticulture and Crop Science, Ohio State University, Columbus, OH 43210. |
Title: | Sources of variation in computer imaging of electrophoretic gels |
Section: | Notes Other records with the "Notes" Section
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Source: | Crop Science. Vol. 38, No. 2, March/April 1998, p. 523-526. |
Publishing Information: | Madison, WI: Crop Science Society of America |
# of Pages: | 4 |
Keywords: | TIC Keywords: Computers; Electrophoresis; Cultivar identification
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Abstract/Contents: | "Electrophoresis is commonly used in agricultural sciences and industries to evaluate protein constituents of plant and animal tissue for cultivar or species identification. Visual analysis of polymorphic bands is effective, but comparison of electrophoretic banding patterns containing common bands of differing optical densities is difficult and subjective. A reliable and reproductive objective means of evaluation is needed. The purpose of this study was to test computer-assisted image analysis for quantitative evaluation of electrophoeretic bands. Seed protein was extracted from mixtures and blends of turfgrass species and cultivars, separated on isoelectric focusing gels, and stained for esterase activity. Banding patterns were scanned and computer-assisted evaluation of band densities performed. Statistical evaluation of repetitive scans of varying band densities revealed that significant variation occurred among gels, scans, and among gel positions. However, when multiple bands from the same sources were averaged across a single gel and evaluated from a single scan, computer-assisted image analysis is an effective quantitative method for evaluation of electrophoretic gels when techniques are used to minimize variation." |
Language: | English |
References: | 10 |
Note: | Figures Tables |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Bell, G. E., M. B. Jr. McDonald, T. K. Danneberger, and S. K. St. Martin. 1998. Sources of variation in computer imaging of electrophoretic gels. Crop Sci. 38(2):p. 523-526. |
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| Web URL(s): https://www.crops.org/publications/cs/pdfs/38/2/CS0380020523 Last checked: 08/05/2010 Requires: PDF Reader Access conditions: Item is within a limited-access website |
| MSU catalog number: SB 183 .C7 |
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