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Publication Type:
| Refereed |
Author(s): | Asano, Y.;
Ito, Y.;
Fukami, M.;
Morifuji, M.;
Fujiie, A. |
Author Affiliation: | Asano and Morifuji: Faculty of Horticulture, Chiba University, Matsudo City, Chiba, Japan; and Ito, Fukami, Fujiie: Chiba Prefectural Agricultural Experiment Station, Chiba City, Chiba, Japan |
Title: | Production of herbicide resistant, transgenic creeping bent plants |
Section: | Genetics and breeding: Contributed papers Other records with the "Genetics and breeding: Contributed papers" Section
|
Meeting Info.: | Sydney, Australia: 1997 |
Source: | International Turfgrass Society Research Journal. Vol. 8, No. Part 1, 1997, p. 261-267. |
Publishing Information: | Blacksburg, VA: International Turfgrass Society |
# of Pages: | 7 |
Keywords: | TIC Keywords: Agrostis stolonifera; Genetic transformation; Bialaphos; Herbicide resistance
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Abstract/Contents: | "We have produced bialaphos resistant, transgenic creeping bent (Agrostis stolonifera 'Penncross') plants by electroporation-mediated direct gene transfer into protoplasts. The protoplasts were isolated from embryogenic suspension cultures that were initiated from seed-derived calli. The plasmids pARK22 and pFFBar containing the bar gene, the expression of which should confer resistance to the phosphinothricin(PPT)-based herbicide to the transformed plants, driven by the CaMV35S promoter and the enhanced CaMV35S promoter, respectively, were used. Electroporation was performed under the conditions of 550 V/cm for 38-40 msec. Electroporated protoplasts were cultured in agarose-solidified, modified K8p medium as a bead-type culture. Bialaphos (2-5 mg/l) selection yielded a total of over 500 resistant colonies from nine independent transformation experiments. Upon transferring the colonies onto gelrite-solidified, regeneration medium, approximately half of them produced plantlets. Most plants that finally grew in soil appeared to have normal morphology. By PCR and PCR-Southern analyses, those plants were proved to be transgenic for the introduced bar gene. The commercial herbicide HerbieTM (= 18% bialaphos) was applied to the transgenic plants. Although the expression levels varied between individuals, all the tested plants showed herbicide resistance at the field application rate of 1%. Both the CaMV35S and the enhanced CaMV35S promoter appeared to be adequate for supporting a sufficient level of expression of the bar gene to produce herbicide resistant, transgenic creeping bent plants." |
Language: | English |
References: | 18 |
Note: | Pictures, b/w Tables |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Asano, Y., Y. Ito, M. Fukami, M. Morifuji, and A. Fujiie. 1997. Production of herbicide resistant, transgenic creeping bent plants. Int. Turfgrass Soc. Res. J. 8(Part 1):p. 261-267. |
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